PDR 2010, 32(6) 495-502 DOI:   10.3724/SP.J.1143.2010.10139  ISSN: 2095-0845 CN: 53-1217/Q

Current Issue | Archive | Search                                                            [Print]   [Close]
muci
Information and Service
This Article
Supporting info
PDF(36150KB)
[HTML]
Reference
Service and feedback
Email this article to a colleague
Add to Bookshelf
Add to Citation Manager
Cite This Article
Email Alert
Keywords
Phaius tankervilliae
Embryo sac
Ultracytochemistry
Potassium antimonite technique
Ca2+
Authors
LI Dong-Mei-1,3
WANG Y-Qin-2
YIE Xiu-Lin-3
PubMed
Article by Li, D. M. 1,
Article by
Article by Wang, Y. Q. 2
Article by Yie, X. L. 3

Ultracytochemical Localization of Calcium during Embryo Sac Development in Phaius tankervilliae(Orchidaceae)

 LI  Dong-Mei-1,3, WANG  Y-Qin-2, YIE  Xiu-Lin-3  

1 Shunde Polytechnic, Foshan 528300, China; 2 College of Life Science, South China
Normal University, Guangzhou 510631, China; 3 South China Botanical Garden,
Chinese Academy of Sciences, Guangzhou 510650, China

Abstract

 Ultracytochemical localization of calcium in embryo sac of Phaius tankervilliae(Aiton) Bl. was carried out using potassium pyroantimonate precipitation method. Results of Ca2+ distribution during embryo sac development are as following: (1) Megasporocyte stage: There is no Ca2+ precipitation in the megasporocyte, nucellar cell and micropyle; (2) Functional megaspore stage: Small particles of Ca2+ precipitates appear on the embryo sac wall at the micropylar end, but no apparent Ca2+ can be found in the functional megaspore; (3) 4nucleate stage: There are significant increase of Ca2+ precipitation on the embryo sac wall and large grains of  the precipitates in the micropyle, while a little starts to appear on the membrane of vacuole; (4) 8nucleate embryo sac stage: Ca2+ precipitation continuously increases on the embryo sac wall. Distribution of Ca2+ in embryo sac shows strong polarity. More Ca2+ precipitations are observed in the synergids and the egg cell than the antipodal cells. Accumulation of Ca2+ precipitation is achieved by (1) transferring Ca2+ to embryo sac through the plasmodesmata between the nucellar cells, which mainly occurred at the chalaza end before the embryo matured; (2) forming large amounts of small bubbles of Ca2+ precipitation, which can cross embryo sac wall to enter embryo sac.

Keywords Phaius tankervilliae   Embryo sac   Ultracytochemistry   Potassium antimonite technique   Ca2+  
Received 2010-07-29 Revised  Online: 2010-10-09 
DOI: 10.3724/SP.J.1143.2010.10139
Fund:

Plan Project sponsored by the Department of Science and Technology of Guangdong Province (2009B020405002)

Corresponding Authors:
Email:
About author:

References:

Antoine AF, Faure JE, Cordeiro S et al, 2000. A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants \
[J\]. Proceedings of the National Academy of Sciences , USA, 97: 10643—10648
Ge LL, Tian HQ, Russell SD, 2007. Calcium function and distribution during fertiliazation in andgiosperms \
[J\]. American Journal of Botany, 94: 1046—1060
Li DM, Ye XL, Liang CY et al, 2006. Growth Path of Pollen Tubes in Ovary of Phaius tankervilliae (Aiton) Bl. (Orchidaceae) \
[J\]. Journal of Tropical and Subtropical Botany, 14 (2): 130—133
Liu XD, Zee SY, Lu YG, 1997. Formation and development of embryo sac wall in rice \
[J\]. Acta Botanica Sinica, 39 (11): 985—990
Mao JQ, Chen YY, Miao Y, 1992. Ca ion localization in the path of pollen tube growth within the gynoecium of Brassica napus\
[J\]. Acta Botanica Sinica, 34 (3): 233—236
Qiu YL, Liu RS, Xie CT et al, 2005,. The dynamic of calcium distribution duing megasporegenesis of Lettuce ( Lactuca sativa L.) \
[J\]. Journal of Plant Physiology and Molecular Biology, 31 (4): 374—382
Qiu YL, Liu RS, Xie CT et al, 2008. Calcium changes during megasporogenesis and megaspore degeneration in lettuce (Lactuma sativa L.) \
[J\]. Sex Plant Reprod, 21: 197—204
Rasmussen H, 1981. Pathways of amplitude and sensitivity modulation in calcium messenger system \
[J\]. FEBS Letters, 30 (4): 1—61
Rasmussen H, Barrett PQ, 1984. Calcium messenger system: An integrated view \
[J\]. Physiological Reviews, 64: 938—998
Tian HQ, Russell SD, 1997. Calcium distribution in fertilized and unfertilized ovules and embryo sacs of Nicotiana tabacum L\
[J\]. Planta, 202: 93—105
Tung SH,Ye XL,Zee SY et al,2000.The microtubular cytoskeleton during megasporogenesis in the Nun orchid, Phaius tankervilliae \
[J\]. New Phytologist,146:503—513
Wei CX, Lan SY, Xu ZX, 2002. Ultracytochemical localization of calcium in the degenerating nucellus and early endosperm cells of rice after flowering \
[J\]. Journal of Chinese Electron Microscopy Society, 21 (1): 34—38
Yang J, Zhao J, Liang SP et al, 2002. Changes of calmodulin distribution in the embryo sac of Oryza sativa before and after fertilization: an immunogold electron microscope study \
[J\]. Acta Botanica Sinica, 44 (3): 264—272
Ye XL, Yeung E, Zee SY et al, 1996. Confocal microscopic observations on microtubular cytoskeleton changes during megasporogenesis and megagametogenesis in Phaius tankervilliae (Aiton) BL. \
[J\]. Acta Botanica Sinica, 38 (9): 677—685
Yu FL, Liang SP, Yang HY, 1998. Ultracytochemical localization of calcium in micropyle and embryo sac of Brassica napus before and after pollination \
[J\]. Acta Botanica Sinica, 40 (7): 591—597
Yu FL, Zhao J, Liang SP et al, 1999. Ultracytochemical localization of calcium in gynoecium and embryo sac of rice \
[J\]. Acta Botanica Sinica, 41 (2): 125—129
Zhang JS, Yong HY, Zhu L et al, 1995. Ultracytochemical localization of calcium in the stigma, style and micropyle of sunflower \
[J\]. Acta Botanica Sinica, 37 (9): 691—696 (in Chinese with English abstract)
Zhang JS, Yong HY, Zhu L et al, 1997. Ultracytochemical localization of calcium in the pollen tube track of cotton gynoecium \
[J\]. Acta Botanica Sinica, 39 (2): 121—125 (in Chinese with English abstract)

Similar articles
1.Xu Hanqing.FORMATION AND DEVELOPMENT 0F POLLEN GRAIN AND EMBRYO SAC OF BASELLA RUBRA[J]. PDR, 1986,8(03): 1-3
2.XING Shu-Ping, XI Xiang-Yuan.AN ULTRASTRUCTURAL STUDY OF EMBRYO SAC IN ARACHIS HYPOGAEA BEFORE AND AFTER FERTILIZATION[J]. PDR, 1996,18(01): 1-3
3.WANG Yin-Zheng LI Jun LIANG Han-Xing WU Zheng-Yi.Embryology of Whytockia hekouensis(Gesneriaceae)[J]. PDR, 2001,23(01): 1-3
4.LIU Lin1 , 2 , YE Xiu-Lin2.

Study on Degeneration of Unfertilized Aposporous Embryo Sac in Pennisetum squamulatium (Gramineae)

[J]. PDR, 2007,29(04): 418-422

Comment for this article:

Copyright by PDR