Chinese Bulletin of Botany ›› 2008, Vol. 25 ›› Issue (05): 591-596.
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Qin Wei1*, Li Wang2, Tihua Fu2, Lijun Zhou1
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Abstract: Well-growing and friable calli from leaves of Cinnamomum longepaniculatum were screened on Murashige and Skoog (MS) medium with different plant growth regulators including 6-benzyladenine (6-BA) and 1-naphthaleneacetic acid (NAA). Calli were cultured on MS medium, Gamborg medium (B5) and woody plant medium (WPM) through suspension culture, and secondary metabolism products were detected. Well-growing and friable-structured calli were induced on 2 mg.L-1 6-BA + 0.5 mg.L-1 NAA, and growth of calli was best on B5 medium in three liquid media. Single cells were formed on B5 medium with 0.2 mg.L-1 6-BA + 0.5 mg.L-1 NAA after two periods of subcultivation. More than 50% benzenemethanol was detected in the secondary metabolism products.
Qin Wei*;Li Wang;Tihua Fu;Lijun Zhou. Studies of Cell Suspension Culture and Induction of Secondary Metabolism Products in Cinnamomum longepaniculatum[J]. Chinese Bulletin of Botany, 2008, 25(05): 591-596.
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