PDR 2011, 33(5) 477- DOI:   10.3724/SP.J.1143.2011.11017  ISSN: 2095-0845 CN: 53-1217/Q

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Karyotype evolution
Secondary constriction
The AI index
Wan Juan
ZHOU Song-Dong
Article by Wan, J.
Article by Zhou, S. D.

Karyotypes of Twenty-five Populations of Thirteen Species in Nomocharis and Lilium

 Wan  Juan, ZHOU  Song-Dong

四川大学生命科学学院,四川 成都610064


Karyotypes of 11 populations of 6 species in Nomocharis and 14 populations of 7 species in Lilium were analyzed to discuss the taxonomic treatment of Nomocharis and elucidate the phylogenetic relationship between Nomocharis and Lilium. Cytological observations were carried out exclusively from root tips. For mitotic arrest, meristematic tissues were pre-treated with 1∶1 mixture of 0.1% colchicine and p-dichlorobenzene at ambient temperature (10℃-20℃) for 8-10h before fixation in Carnoy I (one part glacial acetic acid to three parts absolute ethanol) at 4℃ for 2-24h. They were macerated in 1mol·L-1 HCl at 60℃ for 5-6 minutes, stained and squashed in Carbol Fuchisn. Karyotype asymmetry was assessed by four indices (As.K%, AI, CVCL and CVCI) in which the later three were newly proposed and had be considered to have phylogenetic significance. Furthermore, we tried to clarify the relationship between Nomocharis and Lilium with the scatter diagram of CVCL against CVCI. The results and conclusions were as follows:1) Karyotypes showed differences among species and populations, especially in the number and location of secondary constrictions as well as the presence or absence of B chromosomes;2) Nomocharis and Lilium are closely related;3) Chromosome structural variation produced abundant types (five in total) of secondary constrictions, which was considered to be the main driving force of evolution in the genus Nomocharis;4) Aneuploid, triploid, B chromosomes and secondary constriction type Ⅲ emerged accompanied by the dispersal of Nomocharis to adjacent areas from its origin and differentiation center, namely, Gaoligongshan. In this paper, the triploid of N.basilissa and karyotypes of L.xanthellum were documented for the first time.

Keywords Karyotype evolution   Nomocharis   Lilium   Secondary constriction   The AI index  
Received 2011-01-25 Revised  Online: 2011-04-06 
DOI: 10.3724/SP.J.1143.2011.11017

国家自然科学基金资助项目(31100161, 31070166);教育部博士点基金资助项目(20090181110064);中国科学院大科学装置开放研究项目(2009-LSF-GBOWS-01);科技部科技基础性工作专项重点项目(2007FY110100)

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