PDR 2011, 33(5) 535-539 DOI:   10.3724/SP.J.1143.2011.11004  ISSN: 2095-0845 CN: 53-1217/Q

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Keywords
Sinomanglietia glauca
Microsatellite
Molecular marker
Transferability
Authors
XIONG Min-1
WANG Jing-1
ZHANG Zhi-Rong-3
ZHANG Zhi-Yong-1、2
PubMed
Article by Xiong, M. 1
Article by Wang, J. 1
Article by Zhang, Z. R. 3
Article by Zhang, Z. Y. 1、2

The Development of Nuclear Microsatellite Markers for an Endangered Plant, Sinomanglietia glauca (Magnoliaceae)

 XIONG  Min-1, WANG  Jing-1, ZHANG  Zhi-Rong-3, ZHANG  Zhi-Yong-1、2

1 Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China; 2 Key State Laboratory
of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3 China Germplasm Bank of Wild Species/Key Laboratory of Biodiversity and Biogeography,
Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China

Abstract

Sinomanglietia glauca is an endangered species found in Yichun of Jiangxi Province and Yongshun of Hunan Province. To further explore its population genetic characteristics, we developed seven microsatellite markers from S.glauca using a FIASCO (fast isolation by AFLP sequences containing repeats) method. The number of alleles (NA) ranged from two to seven in 16 samples of four wild populations. The ranges of observed (HO) and expected (HE) heterozygosities were 0.079-0.989 and 0.187-0.600, respectively. The transferability of the seven microsatellite loci was tested upon nine related species of S.glauca within Magnoliaceae. Among which, two (28.5%, Michelia macclurei) to six (85.7%, Tsoongiodendron odorum) loci were successfully amplified. These polymorphic microsatellite markers could be used to decipher the genetic structure, breeding system and population history of S.glauca as well as other relatives within Magnoliaceae.

Keywords Sinomanglietia glauca   Microsatellite   Molecular marker   Transferability  
Received 2011-01-07 Revised  Online: 2011-03-03 
DOI: 10.3724/SP.J.1143.2011.11004
Fund:

江西省自然科学基金项目(2009GZN0016);系统与进化植物学国家重点实验室开放课题;国家自然科学基金(30760016)

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