Abstract: Aim:To optimize the factors that affect transformation system of tomato cotyledon explant.Method:By orthogonal design of statistics,factors that affect transformation such as the Agrobacterium concentration,infiltration time and transform style were assembled.Result:Best transformation system could be set up by the contidition of 2 days preculture,30-fold-dilution,10-minute-infiltation of Agrobacterium solution,and 2 days co-cultivation.The frenquency of success transformation reached to 25%,higher than average level at present.Conclusion:Factors that affect transformation system of tomato cotyledon explant were successfully optimized.

Key words: Tomato；Cotyledon explant；Transformation system