Abstract: From summer harvested roots of 2-4 years old plants of Pokeweed, two antifungal proteins named PAFP-R1 and PAFP-R2 were isolated by using a procedure including extrac-tion with saline solution, purification with ion exchange chromatography on CM-sephadex, gel filtration on sephadex G50 and chromatography on hydroxyapatite. Both proteins at 0.1 mg/ml inhibited obviously the growth of Trichoderma viride mycelia on a PGA medium plates, but even at 1 mg / ml did not inhibit the multiplication of bacteria E. cola and B. subtilis. They are both a single polypeptide with Mr of 13 kD and 15 kD respectively, with a pi of about 5.8 and with Ala as the N-terminus.

Key words: Phytolacca americana(Pokeweed