PDR 2013, 35(5) 555-562 DOI:   10.7677/ynzwyj201312130  ISSN: 2095-0845 CN: 53-1217/Q

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Anemoclema glaucifolium
Genetic diversity
Conservation genetics
GUAN Meng-Meng-1、2
MA Rui-1
GONG Xun-1
Article by Guan, M. M. 1、2
Article by Ma, R. 1
Article by Gong, X. 1

Conservation Genetics of an Endemic Plant, Anemoclema glaucifolium, in the Jinsha River Valley

 GUAN  Meng-Meng-1、2, MA  Rui-1, GONG  Xun-1

1 Key Laboratory of Economic Plant and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650201, China; 2 University of Chinese of Sciences, Beijing 100049, China


 Anemoclema W.T. Wang, a monotypic genus of Ranunculaceae, is endemic to the Jinsha River Valley in southwest China. Combining field investigation with molecular marker analysis (SNPs), we investigated the genetic diversity and genetic structure of A.glaucifolium. Analysis of three chloroplast DNA (cpDNA) regions (rps16 intron, psbAtrnH intergenic spacer and trnCycf6 intergenic spacer) revealed a low level of genetic diversity within the species, but high divergence among populations. This genetic structure is possibly caused by a long period of historical geographic isolation, a relatively narrow distribution range and limited gene flow due to habitat fragmentation. Hydropower stations are scheduled to be built in the Jinsha River drainage system in areas that overlap the range of A.glaucifolium and as a result of their construction the habitats of A.glaucifolium will be flooded or adversely affected in other ways, thus threatening the survival of the species. The results of our analysis of genetic diversity in A.glaucifolium are of value for developing an appropriate conservation strategy for this vulnerable species.

Keywords Anemoclema glaucifolium   cpDNA   SNPs   Genetic diversity   Conservation genetics  
Received 2012-10-25 Revised  Online: 2013-01-15 
DOI: 10.7677/ynzwyj201312130
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Arthur JL, Camm JD, Haight RG et al., 2004. Weighing conservation objectives: maximum expected coverage versus endangered species protection[J]. Ecological Applications, 14 (6): 1936—1945
Caicedo AL, Schaal BA, 2004. Population structure and phylogeography of Solanum pimpinellifolium inferred from a nuclear gene[J]. Molecular Ecology, 13 (7): 1871—1882
Caughley G, Gunn A, 1996. Conservation Biology in Theory and Practice[M]. Oxford: Blackwell Science Inc
Chen S, Wu G, Zhang D et al., 2008. Potential refugium on the QinghaiTibet Plateau revealed by the chloroplast DNA phylogeography of the alpine species Metagentiana striata (Gentianaceae) [J]. Botanical Journal of the Linnean Society, 157 (1): 125—140
Clement M, Posada D, Crandall KA, 2000. TCS: a computer program to estimate gene genealogies[J]. Molecular Ecology, 9 (10): 1657—1659
Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J]. Phytochemical Bulletin, 19 (1): 11—15
Excoffier L, Laval G, Schneider S, 2005. Arlequin (version 3.0): an integrated software package for population genetics data analysis[J]. Evolutionary Bioinformatics Online, 1: 47—50
Excoffier L, Smouse PE, Quattro JM, 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data[J]. Genetics, 131 (2): 479—491
Frankham R, Ballou JD, Briscoe DA, 2002. Introduction to Conservation Genetics[M]. Britain: Cambridge University Press
Hall TA, 1999. BioEdit: a userfriendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95—98
Hamrick JL, Godt MJW, ShermanBroyles SL, 1992. Factors influencing levels of genetic diversity in woody plant species[J]. New Forests, 6 (1): 95—124
Hedrick PW, Miller PS, 1992. Conservation genetics: techniques and fundamentals[J]. Ecological Applications, 2 (1): 30—46
Jin ZZ (金振洲), 2002. Floristic Features of DryHot and DryWarm Valleys in Yunnan and Sichuan (滇川干热河谷与干暖河谷植物区系特征) [M]. Kunming: Yunnan Science and Technology Press
Jin ZZ, Ou XK, Ou PD et al., 1994. A preliminary study on the floristic characteristics of seed plants in the dryhot river valley of JinShaJiang[J]. Acta Botanica Yunnanica (云南植物研究), 16 (1): 1—16
Li XW, Li J, 1993. A preliminary floristic study on the seed plants from the region of Hengduan Mountain[J]. Acta Botanica Yunnanica (云南植物研究), 15 (3): 217—231
Liu L, 1980. Flora of China (Vol.28) [M]. Beijing: Science press
Marshall D, Brown A, 1975. Optimum sampling strategies in genetic conservation[J]. Crop Genetic Resources for Today and Tomorrow, 53—80
Mu J, Li ZB, Li P et al., 2010. A study on vegetation restoration technology of abandoned dreg site of hydropower station in the dry hot valley areas[J]. Journal of Basic Science and Engineering, 18: 245—251
Myers N, Mittermeier RA, Mittermeier CG et al., 2000. Biodiversity hotspots for conservation priorities[J]. Nature, 403 (6772): 853—858
Nei M, Li WH, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proceedings of the National Academy of Sciences of USA, 76 (10): 5269
Nei M, Tajima F, 1983. Maximum likelihood estimation of the number of nucleotide substitutions from restriction sites data[J]. Genetics, 105 (1): 207
Petit RJ, 1999. Diversitégénétique et histoire des populations d’arbres forestiers[J]. Dossier d’habilitaion à diriger des recherches, Université de ParisSud, Université Formation de Recherche Scientifique d’Orsay, Paris, France
Rogers AR, Harpending H, 1992. Population growth makes waves in the distribution of pairwise genetic differences[J]. Molecular Biology and Evolution, 9 (3): 552—569
Rozas J, SánchezDelBarrio JC, Messeguer X et al., 2003. DnaSP, DNA polymorphism analyses by the coalescent and other methods[J]. Bioinformatics, 19 (18): 2496—2497
Templeton AR, Sing CF, 1993. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping. IV. Nested analyses with cladogram uncertainty and recombination[J]. Genetics, 134 (2): 659—669
Thompson JD, Gibson TJ, Plewniak F et al., 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Research, 25 (24): 4876—4882
Wakeley J, 2008. Coalescent Theory[M]. USA: Roberts & Company
Wang W, Lu AM, Ren Y et al., 2009. Phylogeny and classification of Ranunculales: Evidence from four molecular loci and morphological data[J]. Perspectives in Plant Ecology, Evolution and Systematics, 11 (2): 81—110
Wang Y, Wu JQ, Tao Y et al., 2003. Natural distribution and ex situ conservation of endemic species Myricaria laxiflora in waterlevelfluctuation zone within ThreeGorges Reservoir area of Changjiang river[J]. Jounal of Wuhan Botanical Research (武汉植物学研究), 21 (5): 415—422
Wright S, 1931. Evolution in Mendelian populations[J]. Genetics, 16 (2): 97
Wu JQ, Zhao ZE, Jin YX et al., 1998. Investigation and study on the endemic plant Myricaria laxiflora in the ThreeGorge reservoir area[J]. Jounal of Wuhan Botanical Research (武汉植物学研究), 16 (2): 111—116
Young A, Boyle T, Brown T, 1996. The population genetic consequences of habitat fragmentation for plants[J]. Trends in Eco
logy & Evolution, 11 (10): 413—418
Zhang GL, Gong X, 2002. The karyotype analysis of Anemoclema glaucifolium and Heteroplexis microcephala both endimic to China[J]. Acta Botanica Yunnanica (云南植物研究), 24 (6): 765—768

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