PDR 2013, 35(5) 555-562 DOI:   10.7677/ynzwyj201312130  ISSN: 2095-0845 CN: 53-1217/Q

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Keywords
Anemoclema glaucifolium
cpDNA
SNPs
Genetic diversity
Conservation genetics
Authors
GUAN Meng-Meng-1、2
MA Rui-1
GONG Xun-1
PubMed
Article by Guan, M. M. 1、2
Article by Ma, R. 1
Article by Gong, X. 1

Conservation Genetics of an Endemic Plant, Anemoclema glaucifolium, in the Jinsha River Valley

 GUAN  Meng-Meng-1、2, MA  Rui-1, GONG  Xun-1

1 Key Laboratory of Economic Plant and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650201, China; 2 University of Chinese of Sciences, Beijing 100049, China

Abstract

 Anemoclema W.T. Wang, a monotypic genus of Ranunculaceae, is endemic to the Jinsha River Valley in southwest China. Combining field investigation with molecular marker analysis (SNPs), we investigated the genetic diversity and genetic structure of A.glaucifolium. Analysis of three chloroplast DNA (cpDNA) regions (rps16 intron, psbAtrnH intergenic spacer and trnCycf6 intergenic spacer) revealed a low level of genetic diversity within the species, but high divergence among populations. This genetic structure is possibly caused by a long period of historical geographic isolation, a relatively narrow distribution range and limited gene flow due to habitat fragmentation. Hydropower stations are scheduled to be built in the Jinsha River drainage system in areas that overlap the range of A.glaucifolium and as a result of their construction the habitats of A.glaucifolium will be flooded or adversely affected in other ways, thus threatening the survival of the species. The results of our analysis of genetic diversity in A.glaucifolium are of value for developing an appropriate conservation strategy for this vulnerable species.

Keywords Anemoclema glaucifolium   cpDNA   SNPs   Genetic diversity   Conservation genetics  
Received 2012-10-25 Revised  Online: 2013-01-15 
DOI: 10.7677/ynzwyj201312130
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