PDR 2014, 36(01) 41-46 DOI:     ISSN: 2095-0845 CN: 53-1217/Q

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Keywords
Microsatellite markers
Rhododendron spinuliferum
Rhododendron spiciferum
FIASCO
Polymorphism
Authors
YAN Li-Jun-1、2、3
ZHANG Zhi-Rong-2
LI De-Zhu-1、2
GAO Lian-Ming-1
PubMed
Article by Yan, L. J. 1、2、3
Article by Zhang, Z. R. 2
Article by Li, D. Z. 1、2
Article by Gao, L. M. 1

Isolation and Characterization of Microsatellite Markers for the Chinese Endemic Species Rhododendron spinuliferum (Ericaceae)

 YAN  Li-Jun-1、2、3, ZHANG  Zhi-Rong-2, LI  De-Zhu-1、2, GAO  Lian-Ming-1

1 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650201, China; 2 Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650201, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China

Abstract

Rhododendron spinuliferum (Ericaceae) is an ornamental and medicinal plant endemic to southwest China. In order to study hybridization between Rspinuliferum and Rspiciferum, a FIASCO (Fast Isolation by AFLP of Sequences Containing Repeats) method was used to develop microsatellite markers in Rspinuliferum. A total of 28 microsatellite markers were isolated from 100 SSR primer pairs, of which 22 were polymorphic. Polymorphism of the 22 polymorphic loci was assessed separately in 24 individuals collected from two wild populations. The number of alleles per locus ranged from 2 to 5, with an average of 34, while observed (HO) and expected (HE) heterozygosities varied from 0083 to 0792 and from 0153 to 0744, respectively. The same 28 microsatellite markers were also tested in two wild populations (12 individuals from each) of Rspiciferum. Twenty two of the markers were successfully amplified, of which 20 were polymorphic. Estimates of diversity in two natural populations of Rspiciferum based on the 20 polymorphic markers revealed that number of alleles per locus ranged from 2 to 6, with a mean of 32, while observed (HO) and expected (HE) heterozygosities ranged from 0000 to 0833 and from 0117 to 0736, respectively. These newly developed microsatellite markers will be used in future studies of hybridization and the population genetics of Rspinuliferum and its closely related species.

Keywords Microsatellite markers   Rhododendron spinuliferum   Rhododendron spiciferum   FIASCO   Polymorphism  
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References:

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