Plant Diversity ›› 2011, Vol. 33 ›› Issue (6): 645-652.doi: 10.3724/SP.J.1143.2011.11022

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 GUO  Thao-1, LI  Wan-Sha-2, ZHA  Xiang-Dong-1   

  1. 1 Schoot of Life Science, Anhui University, Hefei 230031, China; 2 Kunming Institute of Botany,
    Chinese Academy of Sciences, Kunming 650201, China
  • Received:2011-02-01 Online:2011-12-25 Published:2011-03-18
  • Supported by:

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Abstract:

Columbia wild type Arabidopsis thaliana was used as experimental material, an activation tagging mutant library was constructed by floral dip method, Agrobacterium tumefaciens with an activation tagging vector pCB260 was transformed into the plant. By mutant screening and phenotypic analysis, two mutants were isolated. The hypocotyl elongation is weak inhibited in the mutant seedlings by continuous white lights and the TDNA flanking sequences were obtained by TAILPCR. The NCBI sequence alignment indicates that TDNA inserted in the first and third exon positions of Cry1, respectively. Mutant phenotype analysis and PCR identification results show that TDNA insertion disrupt the function of CRY1 and abolish the process of plant photomorphogenesis.

Key words: Arabidopsis thaliana, Mutant, TAIL-PCR, CRY1

CLC Number: 

  • Q 78