ST273 Is Essential for Tapetum and Microspore Development in Arabidopsis thaliana*

doi:10.3724/SP.J.1143.2012.12052

Abstract

Abstract:

In Arabidopsis, the tapetum plays important roles in anther and pollen development by providing enzymes for callose dissolution, materials for pollenwall formation, and nutrients for microspore development. This paper describes the functional analyses of the ST273 gene in anther and pollen development by using Arabidopsis male sterile mutant st273. Mutant st273 was identified from a TDNA insertion mutant population, and genetic analysis showed that st273 mutant was controlled by a single recessive nuclear gene. A mapbased cloning approach was used, and ST273 gene was mapped to be linked to a molecular marker CIW11 on chromosome 3. Bioinformatics analysis revealed that there is a TDF1 gene near the marker CIW11. Sequencing analysis indicated that st273 mutant had a G459 to A459 basepair change in the third exon of TDF1 gene, which resulted in premature termination mutation in this region. Allelism test indicated that ST273 and TDF1 belong to the same locus. The mutant plant grows normally during the vegetative growth stage, but show developmental defects at the reproductive growth stage. Alexander staining showed that there was no pollen in the mature anther locule. Cytology observation indicated that the mutant tapetum was enlarged and vacuolated, the tetrads could not release the microspores timely, and finally no pollen was formed in the anther. These results demonstrated that ST273 protein plays an important role in tapetum and microspore development.

Key words: Arabidopsis thaliana, Male sterile, Anther development, Tapetum, ST273

CLC Number:

Q 75
Q 945

CHENG Zhi-Peng, CHANG Hai-Shuang, YANG Zhong-Nan, ZHANG Sen. ST273 Is Essential for Tapetum and Microspore Development in Arabidopsis thaliana*[J]. Plant Diversity, 2012, 34(5): 502-508.

References

Alexander MP, 1969. Differential staining of aborted and nonaborted pollen［J］. Stain Technology, 44: 117—122
Albrecht C, Russinova E, Hecht V et al., 2005. The Arabidopsis thaliana SOMATIC EMBRYOGENESIS RECEPTORLIKE KINASES 1 and 2 control male sporogenesis［J］. The Plant Cell, 17: 3337—3349
Berger F, Taylor A, Brownlee C, 1994. Cell fate determination by the cell wall in early Fucus development［J］. Science, 263: 1421—1423
Bonner L, Dickinson H, 1989. Anther dehiscence in Lycopersicon esculentum Mill［J］. New Phytologist, 113 (1): 97—115
Canales C, Bhatt AM, Scott R et al., 2002. EXS, a putative LRR receptor kinase, regulates male germline cell number and tapetal identity and promotes seed development in Arabidopsis［J］. Current Biology, 12: 1718—1727
Colcombet J, BoissonDernier A, RosPalau R et al., 2005. Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASES 1 and 2 are essential for tapetum development and microspore maturation［J］. The Plant Cell, 17: 3350—3361
Ding B, 1997. Cell to cell transport of macromolecules through plasmadesmata: a novel signaling pathway in plants［J］. Trends Cell Biology, 7: 5—9
Goldberg RB, Beals TP, Sanders PM, 1993. Anther development: basic principles and practical applications［J］. The Plant Cell, 5: 1217—1229
Higginson T, Li SF, Parish RW, 2003. AtMYB103 regulates tapetum and trichome development in Arabidopsis thaliana［J］. The Plant Journal, 35: 177—192
Hird DL, Worrall D, Hodge R et al., 1993. The antherspecific protein encoded by the Brassica napus and Arabidopsis thaliana A6 gene displays similarity to b1, 3glucanase［J］. The Plant Journal, 4: 1023—1033
Hord CL, Chen C, DeYoung BJ et al., 2006. The BAM1/BAM2 receptorlike kinases are important regulators of Arabidopsis early anther development［J］. The Plant Cell, 18: 1667—1680
Ito T, Nagata N, Yoshiba Y et al., 2007. Arabidopsis MALE STERILITY 1 encodes a PHDtype transcription factor and regulates pollen and tapetum development［J］. The Plant Cell, 19: 3549—3562
Li SF, Higginson T, Parish RW, 1999. A novel MYBrelated gene from Arabidopsis thaliana expressed in development anthers［J］. Plant Cell Physiology, 40: 343—347
Mariani C, De Beuckeleer M, Truettner J et al., 1990. Induction of male sterility in plants by achimeric ribonuclease gene［J］. Nature, 347: 737—741
Millar AA, Gubler F, 2005. The Arabidopsis GAMYBlike genes, MYB33 and MYB65, are microRNAregulated genes that redundantly facilitate anther development［J］. The Plant Cell, 17: 705—721
Pacini E, Franchi GG, Hesse M, 1985. The tapetum: its form, function, and possible phylogeny in Embryophyta［J］. Plant Systematics and Evolution, 149: 155—185
Piffanelli P, Ross JHE, Murphy DJ, 1998. Biogenesis and function of the lipidic structures of pollen grains［J］. Sexual Plant Reproduction, 11: 65—80
Sanders PM, Bui AQ, Goldberg RB, 1999. Anther developmental defects in Arabidopsis thaliana malesterile mutants［J］. Sexual Plant Reproduction, 11: 297—322
Schiefthaler U, Balasubramanian S, Sieber P et al., 1999. Molecular analysis of NOZZLE, a gene involved in pattern formation and early sporogenesis during sex organ development in Arabidopsis thaliana［J］. Proceedings of the National Academy of Sciences, USA, 96: 11664—11669
Sorensen A, Krober S, Unte US et al., 2003. The Arabidopsis ABORTED MICROSPORES (AMS) gene encodes a MYC class transcription factor［J］. The Plant Journal, 33: 413—423
Stieglitz H, Stern H, 1973. Regulation of β1, 3glucanase activity in developing anthers of Lilium［J］. Developmental Biology, 34: 169—173
Strauss E, 1998. When wall can talk, plant biologist listen［J］. Science, 282: 28—29
Sun Y, Zhang W, Li FL et al., 2000. Identification and genetic mapping of four novel genes that regulate leaf development in Arabidopsis［J］. Cell Research, 10 (4): 325—335
Wilson ZA, Morroll SM, Dawson J et al., 2001. The Arabidopsis MALE STERILITY 1 (MS1) gene is a transcriptional regulator of male gametogenesis, with homology to the PHDfinger family of transcription factors［J］. The Plant Journal, 28: 27—39
Wolpert L, 1996. One hundred years of positional information［J］. Trends Genet, 12: 359—364
Yang C, VizcayBarrena G, Conner K et al., 2007. MALE STERILITY 1 is required for tapetal development and pollen wall biosynthesis［J］. The Plant Cell, 19: 3530—3548
Yang SL, Xie LF, Mao HZ et al., 2003. TAPETUM DETERMINANT 1 is required for cell specialization in the Arabidopsis anther［J］. The Plant Cell, 15: 2792—2804
Yang WC, Ye D, Xu J et al., 1999. The SPOROCYTELESS gene of Arabidopsis is required for initiation of sporogenesis and encodes a novel nuclear protein［J］. Genes and Development, 13: 2108—2117
Yi J (易君), Gao JF (高菊芳), Zhang ZB (张在宝) et al., 2006. Genetic and mapping analysis of Arabidopsis thaliana male sterile mutant ms1502 (Cruciferae) ［J］. Acta Botanica Yunnanica (云南植物研究), 28 (3): 283—288
Zhang W, Sun YJ, Timofejeva L et al., 2006. Regulation of Arabidopsis tapetum development and function by DYSFUNCTIONAL TAPETUM1 (DYT1) encoding a putative bHLH transcription factor［J］. Development, 133: 3085—3095
Zhang ZB, Zhu J, Gao JF et al., 2007. Transcription factor AtMYB103 is required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis［J］. Plant Journal, 52: 528—538
Zhao DZ, Wang GF, Speal B et al., 2002. The EXCESS MICROSPOROCYTES 1 gene encodes a putative leucinerich repeat receptor protein kinase that controls somatic and reproductive cell fates in the Arabidopsis anther［J］. Genes and Development, 16: 2021—2031
Zhu J, Chen H, Li H et al., 2008. Defective in tapetal development and function 1 is essential for anther development and tapetal function for microspore maturation in Arabidopsis［J］. The Plant Journal, 55: 266—277

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