PDR 2014, 36(04) 443-448 DOI:    10.7677/ynzwyj201413191  ISSN: 2095-0845 CN: 53-1217/Q

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Keywords
Castanopsis tibetana
Microsatellite
Transferability
Genetic diversity
Authors
YE Lin-Jiang
WANG Jing
SUN Peng
DONG Shu-Peng
ZHANG Zhi-Yong
PubMed
Article by Ye, L. J.
Article by Wang, J.
Article by Sun, P.
Article by Dong, S. P.
Article by Zhang, Z. Y.

The Transferability of Nuclear Microsatellite Markers in Four Castanopsis Species to Castanopsis tibetana (Fagaceae)

 YE  Lin-Jiang, WANG  Jing, SUN  Peng, DONG  Shu-Peng, ZHANG  Zhi-Yong

Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China

Abstract

Castanopsis tibetana is a typical and dominant component in subtropical evergreen broadleaved forests of China. However, its genetic diversity and longterm persistence have been jeopardized by habitat fragmentation. To examine its genetic diversity and genetic structure, crossspecies amplification of 51 microsatellite loci which are derived from four congeneric species, ie., Castanopsis chinensis, Csclerophylla, Ccuspidata and Csieboldii, were tested in Ctibetana.Thirtyfive loci were successfully amplified in Ctibetana, among them 11 were polymorphic across four wild populations of Ctibetana (24 individuals in total). Genetic diversity of four Ctibetana populations (Jiangxi)  was relatively low. The number of alleles (NA) ranged from 2 to 7 (average 45) and the ranges of observed (HO) and expected (HE) heterozygosities were 0505 and 0471,respectively. The results will facilitate the study and conservation of genetic diversity of Ctibetana.

Keywords Castanopsis tibetana   Microsatellite   Transferability   Genetic diversity  
Received 2013-09-19 Revised  Online: 2014-03-25 
DOI: 10.7677/ynzwyj201413191
Fund:

国家自然科学基金 (31160043, 31160082);国家科技支撑计划 (2012BAC11B02)

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