PDR 2014, 36(04) 443-448 DOI:    10.7677/ynzwyj201413191  ISSN: 2095-0845 CN: 53-1217/Q

Current Issue | Archive | Search                                                            [Print]   [Close]
Information and Service
This Article
Supporting info
Service and feedback
Email this article to a colleague
Add to Bookshelf
Add to Citation Manager
Cite This Article
Email Alert
Castanopsis tibetana
Genetic diversity
YE Lin-Jiang
SUN Peng
DONG Shu-Peng
ZHANG Zhi-Yong
Article by Ye, L. J.
Article by Wang, J.
Article by Sun, P.
Article by Dong, S. P.
Article by Zhang, Z. Y.

The Transferability of Nuclear Microsatellite Markers in Four Castanopsis Species to Castanopsis tibetana (Fagaceae)

 YE  Lin-Jiang, WANG  Jing, SUN  Peng, DONG  Shu-Peng, ZHANG  Zhi-Yong

Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China


Castanopsis tibetana is a typical and dominant component in subtropical evergreen broadleaved forests of China. However, its genetic diversity and longterm persistence have been jeopardized by habitat fragmentation. To examine its genetic diversity and genetic structure, crossspecies amplification of 51 microsatellite loci which are derived from four congeneric species, ie., Castanopsis chinensis, Csclerophylla, Ccuspidata and Csieboldii, were tested in Ctibetana.Thirtyfive loci were successfully amplified in Ctibetana, among them 11 were polymorphic across four wild populations of Ctibetana (24 individuals in total). Genetic diversity of four Ctibetana populations (Jiangxi)  was relatively low. The number of alleles (NA) ranged from 2 to 7 (average 45) and the ranges of observed (HO) and expected (HE) heterozygosities were 0505 and 0471,respectively. The results will facilitate the study and conservation of genetic diversity of Ctibetana.

Keywords Castanopsis tibetana   Microsatellite   Transferability   Genetic diversity  
Received 2013-09-19 Revised  Online: 2014-03-25 
DOI: 10.7677/ynzwyj201413191

国家自然科学基金 (31160043, 31160082);国家科技支撑计划 (2012BAC11B02)

Corresponding Authors:
About author:


邹喻苹, 葛颂, 王晓东, 2001. 系统与进化植物学中的分子标记[M]. 北京: 科学出版社
徐永椿, 任宪威, 1985. 壳斗科[A]. 见: 郑万钧, 中国树木志 第2卷[M]. 北京: 中国林业出版社, 2198—2199; 2324—2357
Barbara T, PalmaSilva C, Paggi GM et al., 2007. Crossspecies transfer of nuclear microsatellite markers: potential and limitations[J]. Molecular Ecology, 16 (18): 3759—3767
Chen FC, Li WH, 2001. Genomic divergences between humans and other hominoids and the effective population size of the common ancestor of humans and chimpanzees[J]. The American Journal of Human Genetics, 68 (2): 444—456
Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J]. Phytochemical Bulletin, 19: 11—15
Farm K, Garden B, 2003. Report of rapid biodiversity assessments at Jiulianshan Nature Reserve, south Jiangxi, China, 2000, 2001 and 2003[J]. South China Forest Biodiversity Survey Report Series, 33: 1—19 (Hong Kong)
Guichoux E, Lagache L, Wagner S et al., 2011. Current trends in microsatellite genotyping[J]. Molecular Ecology Resources, 11 (4): 591—611
He P (何平), 1998. Abundance, polymorphism and applications of microsatellite in eukaryote[J]. Hereditas (遗传), 20 (4): 42—47
Huang GM, Hong L, Ye WH et al., 2009. Isolation and characterization of polymorphic microsatellite loci in Castanopsis chinensis Hance (Fagaceae) [J]. Conservation Genetics, 10 (4): 1069—1071
Kalia RK, Rai MK, Kalia S et al., 2011. Microsatellite markers: an overview of the recent progress in plants[J]. Euphytica, 177 (3): 309—334
Kuleung C, Baenziger PS, Dweikat I, 2004. Transferability of SSR markers among wheat, rye, and triticale[J]. Theoretical and Applied Genetics, 108 (6): 1147—1150
Lagdgerantz U, Ellegren H, Andersson L, 1993. The abundance of various polymorphic microsatellite motifs differs between plants and vertebrates[J]. Nucleic Acids Research, 21 (5): 1111—1115
Liu MQ (刘孟奇), Zhou ZK (周浙昆), 2006. Modern and geological distribution of Castanopsis (Fagaceae) [J]. Acta Botanica Yunnanica (云南植物研究), 28 (3): 223—235
Li JQ (李建强), 1996. On the phylogeny of the Fagaceae[J]. Acta Phytotaxonomica Sinica (植物分类学报), 34 (6): 597—609
Ni J (倪健), Song YC (宋永昌), 1997. Relationship between climate and distribution of main species of subtropical evergreen broadleaved forest in China[J]. Acta Phytoecologica Sinica (植物生态学报), 21 (2): 115—129
Powell W, Machray GC, Provan J, 1996. Polymorphism revealed by simple sequence repeats[J]. Trends in Plant Science, 1 (7): 215—222
Peakall ROD, Smouse PE, 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research[J]. Molecular Ecology Notes, 6 (1): 288—295
Rassmann K, Schlotterer C, Tautz D, 1991. Isolation of simple sequence loci for use in polymerase chain reactionbased DNA fingerprinting[J]. Electrophoresis, 12 (23): 113—118
Raymond M, Rousset F, 1995. GENEPOP(Version 12): population genetics software for exact tests and ecumenicism[J]. Journal of Heredity, 86 (3): 248—249
Shi YS, Zhang J, Jiang K et al., 2011. Development and characterization of polymorphic microsatellite markers in Castanopsis sclerophylla (Fagaceae) [J]. American Journal of Botany, 98 (2): e19—e21
Ueno S, Yoshimaru H, Kawahara T et al., 2008. Isolation of microsatellite markers in Castanopsis cuspidata var. sieboldii Nakai from an enriched library[J]. Molecular Ecology, 9 (8): 1187—1189
Ueno S, Yoshimaru H, Kawahara T et al., 2003. A further six microsatellite markers for Castanopsis cuspidata var. sieboldii Nakai[J]. Conservation Genetics, 4 (6): 813—815
Ueno S, Aoki K, Tsumura Y, 2009. Generation of Expressed Sequence Tags and development of microsatellite markers for Castanopsis sieboldii var. sieboldii (Fagaceae) [J]. Annals of Forest Science, 66 (5): 1—21Wu K, Tanksley SD, 1993. Abundance polymorphism and genetic mapping of microsatellites in rice[J]. Molecular and General Genetics, 24 (12): 225—235Wang Y, Kang M, Huang HW, 2008. Microsatellite loci transferability in chestnut[J]. Journal of the American Society for Horticultural Science, 133 (5): 692—700Young A, Boyle T, Brown T, 1996. The population genetic consequences of habitat fragmentation for plants[J]. Trends in Ecology and Evolution, 11 (10): 413—418Yao X, Zhang J, Ye Q et al., 2008. Characterization of 14 novel microsatellite loci in the endangered Liriodendron chinense (Magnoliaceae) and crossspecies amplification in closely related taxa[J]. Conservation Genetics, 9 (2): 483—485Zane L, Bargelloni L, Patarnello T, 2002. Strategies for microsatellite isolation: a review[J]. Molecular Ecology, 11 (1): 1—16Zhang JS (张嘉生), 2005. Study of Castanopsis tibetana community dominant plant population competition[J]. Journal of Fujian Forestry Science and Technology (福建林业科技), 32 (4): 82—85

Similar articles
1.RUAN Ren-Chao CHEN Hui - Cha ZHANG Zai - Xing YANG Yu- Shun JIN Tao-Ye YOU Jun-Met.Progress and Prospects of Research and Utilization on Genetic Diversity of Rice Germplasm Resources in Guizhou[J]. PDR, 2000,22(12): 1-3
2.LI Qiao-Ming XU Zai-Fu.Genetic Diversity and Population Differentiation of Vatica guangxiensis [J]. PDR, 2001,23(02): 1-3
3.LI Qiao-Ming XU Zai-Fu.Population Genetic Structure and Differentiaton of Parashorea chinensis(Dipterocarpaceae)[J]. PDR, 2001,23(03): 1-3
4.LI Rong - Chun.Analyses on Genetic Diversity of Agaricus bisporus[J]. PDR, 2001,23(04): 1-3
5.PENG Yu-Lan, HU Yun-Qian, SUN Hang.Allozyme Analysis of Davidia involucrata var. vilmoriniana and Its Biogeography Significance[J]. PDR, 2003,25(01): 1-3
6.TIAN Hong-Li; ZHOU Shi-Liang.A Review on Systematics and Genetic Diversity of Nelumbonaceae[J]. PDR, 2006,28(04): 341-348
7.ZHANG Ping; DONG Yu-Zhi; WEI Yan; HU Cheng-zhi.Analysis of Genetic Diversity of Haloxylon persicum (Chenopodiaceae) in Xinjiang by ISSR[J]. PDR, 2006,28(04): 359-362
8.CHENG Shao-Li; LI Zhi-Lin; ZHANG Jun-Xiang; FAN Cheng-Ming; ZHAO Ming-Fu; HE Yue-Qiu.Similarity Analysis of the Main Species of Cymbidium (Orchidaceae) from Yunnan Province Using RAPD Technique[J]. PDR, 2006,28(06): 565-569
9.LIU Li; ZHU Yong-Qing; WANG You-Fang.The Morphological and RAPD Analysis in Different Myuroclada maximowiczii (Brachytheciaceae) Populations[J]. PDR, 2006,28(06): 570-574
10.HE Jun1 , 2 , YANG Bai-Yun1 , CHEN Shao-Feng1 , GAO Lian-Ming2 , WANG Hong2.Assessment of Genetic Diversity of Paris polyphylla (Trilliaceae) by ISSR Markers[J]. PDR, 2007,29(04): 388-392
11.WANG Jin-Feng1 , 2 , Chiaki kuroda3 , GONG Xun1.Assessment of Genetic Variation and Differentiation of Ligularia tongolensis (Compositae) Detected by ISSRs[J]. PDR, 2007,29(05): 537-542
12.PENG Yan-Qiu1, SHAO Jian-Wen1, 2, ZHANG Xiao-Ping1, 2, ZHANG Zhong-Xin1, ZHU Guo-Ping1, 2.Genetic Diversity of the Endangered and Endemic Species, Primula merrilliana (Primulaceae), Revealed by RAPD Analysis[J]. PDR, 2007,29(05): 549-553
13.WANG Shu-Li1 , 2, LI Qiao-Ming1.Population Genetic Diversity of Bombax malabaricum(Bombacaceae) in China[J]. PDR, 2007,29(05): 529-536
14.CAI Ning-Feng1 , 2 , YAN Ning1 , HU Hong1 , LIU Tao3.Genetic Structure and Clonal Diversity of Cypripedium flavum (Orchidaceae) Populations from South-West China[J]. PDR, 2008,30(01): 69-75

Comment for this article:

Copyright by PDR