Chinese Bulletin of Botany ›› 2002, Vol. 19 ›› Issue (05): 584-587.

• 研究论文 • Previous Articles     Next Articles

Rapid Amplification of 3′cDNA End of Suaeda liaotungensis Choline Monooxygenase Using One-step PCR

LI Qiu-Li 2GAO Xiao-RongYUAN Xiao-Dong Liu Da-WeiAN Li-Jia   

  1. 1(Biology Department,Liaoning Normal University, Dalian 116029)2(Bioengineering Department,Dalian University of Technology,Dalian 116012)3(Takara Biotechnology (Dalian) Co.Ltd., Dalian 116600)
  • Received:2001-11-05 Revised:2002-05-08 Online:2002-09-20 Published:2002-09-20
  • Contact: AN Li-Jia

Abstract: A modified method of 3′RACE can rapidly amplify 3′cDNA end by using one-step PCR with a specific primer and Oligo_dT primer. We successfully cloned the 3′cDNA ends of Suaeda liaotungensis choline monooxygenase using this method. Compared with the typical 3′RACE, one_step PCR has the advantages of rapid, efficient and inexpensive.