Chinese Bulletin of Botany ›› 2002, Vol. 19 ›› Issue (05): 584-587.
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LI Qiu-Li 2GAO Xiao-RongYUAN Xiao-Dong Liu Da-WeiAN Li-Jia
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Abstract: A modified method of 3′RACE can rapidly amplify 3′cDNA end by using one-step PCR with a specific primer and Oligo_dT primer. We successfully cloned the 3′cDNA ends of Suaeda liaotungensis choline monooxygenase using this method. Compared with the typical 3′RACE, one_step PCR has the advantages of rapid, efficient and inexpensive.
LI Qiu-Li GAO Xiao-RongYUAN Xiao-Dong Liu Da-WeiAN Li-Jia. Rapid Amplification of 3′cDNA End of Suaeda liaotungensis Choline Monooxygenase Using One-step PCR[J]. Chinese Bulletin of Botany, 2002, 19(05): 584-587.
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