PDR 2015, 37(01) 29-37 DOI:   10.7677/ynzwyj201514048  ISSN: 2095-0845 CN: 53-1217/Q

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Keywords
Camellia sinensis var. assamica
Camellia taliensis
Domestication
Genetic diversity
Microsatellite (SSR)
STRUCTURE clustering
Authors
LI Miao-Miao-1、3
MEEGAHAKUMBURA M. Kasun1、2、3
YAN Li-Jun-1、2、3
LIU Jie-1
GAO Lian-Ming-1
PubMed
Article by Li, M. M. 1、3
Article by MEEGAHAKUMBURA M. Kasun1、2、3
Article by Yan, L. J. 1、2、3
Article by Liu, J. 1
Article by Gao, L. M. 1

Genetic Involvement of Camellia taliensis in the Domestication of C.sinensis var. assamica (Assimica Tea) Revealed by Nuclear Microsatellite Markers

 LI  Miao-Miao-1、3, MEEGAHAKUMBURA  M. Kasun1、2、3, YAN  Li-Jun-1、2、3, LIU  Jie-1, GAO  Lian-Ming-1

1 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650201, China; 2 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese
Academy of Sciences, Kunming 650201, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China

Abstract

The levels of genetic diversity and population structure were assessed for 104 ancient tea plants from three Camellia taliensis populations, three Csinensis var. assamica populations and two transitional populations of Ctaliensis based on data from 11 nuclear microsatellite loci. In this study, a relative low genetic diversity was revealed for all three population groups. The average number of alleles (Na) was 485, the average Shannon’s diversity index (I) was 117, the average expected heterozygosity (He) and observed heterozygosity (Ho) was 059 and 052 respectively, for the studied populations. The level of genetic diversity of Ctaliensis was lower than for Csinensis var. assamica and the transitional Ctaliensis. An AMOVA analysis indicated a significant genetic differentiation (FST=0305) between populations of Csinensis var. assamica and Ctaliensis. Most of the genetic variation was partitioned within population of Csinensis var. assamica (9351%) and Ctaliensis (8941%), and a low partition among populations (649% and 1059%, respectively). Populations of Csinensis var. assamica and Ctaliensis (including the transitional populations of Ctaliensis) formed two distinct genetic clusters in a principal component analysis (PCoA) and in STRUCTURE clustering, which suggests that the transitional populations of Ctaliensis originated mainly from Ctaliensis, and then followed somewhat genetic differentiation during the process of domestication. Gene introgression was detected in the cultivated Csinensis var. assamica and Ctaliensis from the same tea garden, and genetic material of Ctaliensis apparently infiltrated into Csinensis var. assamica. This study demonstrated that Ctaliensis was genetically involved in the domestication of Csinensis var. assamica. Finally, suggestions on how to protect the genetic resources of ancient tea plants are discussed on the findings in this study.

Keywords Camellia sinensis var. assamica   Camellia taliensis   Domestication   Genetic diversity   Microsatellite (SSR)   STRUCTURE clustering  
Received 2014-03-21 Revised  Online: 2014-04-14 
DOI: 10.7677/ynzwyj201514048
Fund:

国家自然科学基金国际 (地区) 合作与交流项目 (31161140350),科技基础性工作专项项目 (2012FY110800)

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