PDR 2015, 37(2) 141-144 DOI:    10.7677/ynzwyj201514063  ISSN: 2095-0845 CN: 53-1217/Q

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Keywords
Nandina domestica
Microsatellite marker
Genetic diversity
Authors
HU Hui-Li
LI Bo
HU Wan
SUN Zhi-Xia
ZHANG Zhi-Yong
FAN Deng-Mei
PubMed
Article by Hu, H. L.
Article by Li, B.
Article by Hu, W.
Article by Sun, Z. X.
Article by Zhang, Z. Y.
Article by Fan, D. M.

Development and Characterization of Microsatellite Loci for Heavenly Bamboo (Nandina domestica)

 HU  Hui-Li, LI  Bo, HU  Wan, SUN  Zhi-Xia, ZHANG  Zhi-Yong, FAN  Deng-Mei

Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China

Abstract

We developed and characterized a total of 10 novel polymorphic microsatellite loci from the genome of an ornamental shrub, Nandina domestica. The number of alleles per locus in 24 individuals from four populations varied from 2 to 6. The expected and observed heterozygosity ranged from 0153 to 0778 and from 0 to 1, respectively. These markers will provide tools for future studies on population genetic diversity of Ndomestica. Additionally, most of our new markers could yield the amplification products in four other Berberidaceae species.

Keywords Nandina domestica   Microsatellite marker   Genetic diversity  
Received 2014-04-10 Revised  Online: 2014-07-20 
DOI: 10.7677/ynzwyj201514063
Fund:

National Natural Science Foundation of China (grant nos. 31160043 and 31160082) and National Science and Technology Support Program (2012BAC11B02)

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References:

Estoup A, Guillemaud T, 2010. Reconstructing routes of invasion using genetic data: why, how and so what?[J]. Molecular Ecology, 19: 4113—4130

Knox GW, Wilson SB, 2006. Evaluating north and south Florida landscape performance and fruiting of ten cultivars and a wildtype selection of Nandina domestica,a potentially invasive shrub[J]. Journal of Environmental Horticulture, 24 (3): 137—142

Raymond M, Rousset F, 1995. GENEPOP: population genetics software for exact tests and ecumenicism, version 12[J]. Journal of Heredity, 86: 248—249

Rychlik W, 2007. OLIGO 7 primer analysis software[J]. Methods in Molecular Biology, 402: 35—59

Ying JS, Boufford DE, Brach AR, 2011. Nandina[A]. // Wu ZY, Raven PH eds., Flora of China[M]. Beijing: Science Press; St. Louis: Missouri Botanical Garden Press, 715

Zane L, Bargelloni L, Patarnello T, 2002. Strategies for microsatellite isolation: a review[J]. Molecular Ecology, 11: 1—16

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