Chin Bull Bot ›› 2013, Vol. 48 ›› Issue (3): 303-312.doi: 10.3724/SP.J.1259.2013.00303

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Cu2+ Induced Local Toxicity and DNA Damage, Cell Death in Roots of Arabidopsis thaliana

Zhiqin Wei1,2†, Zhiyong Chen2†, Rong Qin2, Yutao Wang2, Shaoshan Li2*   

  1. 1School of Life Sciences, Zunyi Normal College, Zunyi 563002, China;

    2Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Sciences, South China Normal University, Guangzhou 510631, China
  • Received:2013-02-01 Revised:2013-04-12 Online:2013-06-21 Published:2013-05-01
  • Contact: Shaoshan Li
  • Supported by:


Abstract: We investigated the effect of different Cu2+ concentrations in the root medium on root growth, reactive oxygen species (ROS) accumulation, antioxidative response, DNA damage, plasma membrane integrity and cell viability in roots of Arabidopsis thaliana, and the toxicity range was explored with split-root technique. Primary root length was significantly inhibited. Cu2+ enhanced the activities of antioxidant enzymes, induced ROS accumulation and DNA damage, and reduced plasma membrane integrity. Cell death was significantly increased with increased concentration of Cu2+. ROS accumulation was significantly and positively correlated with the activities of total superoxide dismutase, catalase, peroxidase and ascorbate peroxidase. ROS accumulation increased linearly with DNA damage, plasma membrane integrity and cell viability. In the split-root experiment, root growth was inhibited in culture medium supplemented with 75 μmol·L–1 Cu2+; however, roots were not inhibited in medium without supplementary Cu2+. ROS accumulation and cell death took place in only half the dish, so Cu2+ toxicity was local, not systemic. Local ROS accumulation in roots likely mediates the cell death caused by Cu2+ stress.

李福燕, 李许明, 赵雄, 杨帆, 张宇, 漆智平 (2010). 热带地区不同水稻品种对土壤镉胁迫的生理生化响应. 生态学杂志 29, 821-825.
聂志刚, 王艳, 李韶山 (2009). 重金属诱导拟南芥原生质体DNA损伤的单细胞凝胶电泳检测. 植物学报 44, 117-123.
王庆仁, 崔岩山, 董艺婷 (2001). 植物修复-重金属污染土壤整治有效途径. 生态学报 21, 326-331.
Atha DH, Wang HH, Petersen EJ, Cleveland D, Holdrook D, Jaruga P, Dizdaroglu M, Xing BS, Nelson Bryant (2012). Copper oxide nanoparticle mediated DNA damage in terrestrial plant models, Environ Sci Technol 46, 1819-1827.
Baker CJ, Mock NM (1994). An improved method for monitoring cell death in cell suspension and leaf disc assays using Evans blue. Plant Cell, Tissue and Organ Cult 39, 7-12.
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities ultilizing the principle of protein-dye binding. Anal Biochem 72, 248-254.
Buckner B, Johal GS, Janick-Buckner D (2000). Cell death in maize. Physiol Plant 108, 231-239.
Cuypers A, Smeets K, Ruytinx J, Opdenakker K, Keunen E, Remans T, Horemans N, Vanhoudt N, Van BF, Guisez Y, Colpaert J, Vangronsveld J (2011). The cellular redox state as a modulator in cadmium and copper responses in Arabidopsis thaliana seedlings. J Plant Physiol 168, 309-316.
Dat J, Vandenabeele SV, Vranová E, Van Montagu M, Inzé D, Van Breusegem F (2000). Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57, 779-795.
Dr??kiewicz M, Skórzyńska-Polit E, Krupa Z (2004). Copper-induced oxidative stress and antioxidant defence in Arabidopsis thaliana. BioMetals 17, 379-387.
Fleury C, Mignotte B, Vayssière JL (2002). Mitochondrial reactive oxygen species in cell death signaling. Biochimie 84, 131-141.
Gansel X, Mu?os S, Tillard P, Gojon A (2001). Differential regulation of the NO3- and NH4+ transporter genes AtNrt2.1 and AtAmt1.1 in Arabidopsis: relation with long-distance and local controls by N status of the plant, Plant J 26, 143-155.
Hattab S, Chouba L, Ben Kheder M, Mahouachi T, Boussetta H (2009). Cadmium- and copper-induced DNA damage in Pisum sativum roots and leaves as determined by the Comet assay. Plant Biosyst 143, S6-S11.
Hung WC, Huang DD, Chien PS, Yeh CM, Chen PY, Chi WC, Huang HJ (2007). Protein tyrosine dephosphorylation during copper-induced cell death in rice roots. Chemosphere 69, 55-62.
Kopittke PM, Menzies NW (2006). Effect of Cu toxicity on growth of cowpea (Vigna unguiculata). Plant Soil 279, 287-296.
Janicka-Russak M, Kabala K, Burzyński M (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J Exp Bot 63, 4133-4142.
Jiang L, Wang Y, Li SS (2007). Application of the comet assay to measure DNA damage induced by UV radiation in the hydrophyte, spirodela polyrhiza. Physiol Plant 129, 652-657.
Jiang L, Wang Y, Bj?rn LO, Li SS (2011). UV-B-induced DNA damage mediates expression changes of cell cycle regulatory genes in Arabidopsis root tips. Planta 233, 831-841.
Lequeux H, Hermans C, Lutts S, Verbruggen N (2010). Response to copper excess in Arabidopsis thaliana: Impact on the root system architecture, hormone distribution, lignin accumulation and mineral profile. Plant Physiol Biochem 48, 673-682.
Li SS, Nie ZZ, Wang Y (2008). Heavy metal induced DNA damage in plant measured by single cell gel electrophoresis. Toxicol Lett, 180S, S200.
Machado MD, Soares EV (2012). Development of a short-term assay based on the evaluation of the plasma membrane integrity of the alga Pseudokirchneriella subcapitata. Appl Microbiol Biotechnol 95, 1035-1042.
Mittler R (2002). Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7, 405-410.
Moura DJ, Péres VF, Jacques RA, Saffi J (2012). Heavy Metal Toxicity: Oxidative Stress Parameters and DNA Repair. In: Gupta DK, Sandalio LM, eds. Metal Toxicity in Plants: Perception, Signaling and Remediation. Berlin: Springer-Verlag. pp. 187-205.
Murphy A, Taiz L (1997). Correlation between potassium efflux and copper sensitivity in 10 Arabidopsis ecotypes. New Phytol 136, 211-222.
Mutlu S, Atici ?, Nalbantoglu B (2009). Effects of salicylic acid and salinity on apoplastic antioxidant enzymes in two wheat cultivars differing in salt tolerance. Biol Plant 53, 334-338.
Ott M, Gogvadze V, Orrenius S, Zhivotovsky B (2007). Mitochondria, oxidative stress and cell death. Apoptosis 12, 913-922.
Ouzounidou G, Mousbakas M, Karataglis S (1995). Responses of maize (Zea mays L.) plants to copper stress: growth, mineral content and ultra structure of roots. Environ Exp Bot 35, 167-176.
Pitzschke A, Forzani C, Hirt H (2006). Reactive oxygen species signaling in plants. Antioxid Redox Signaling 8, 1757-1764.
Rana SVS (2008). Metals and apoptosis: Recent developments. J Trace Elem Med Biol 22, 262-284.
Schützendübel A, Polle A (2002). Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot 53, 1351-1365.
Tang B, Xu SZ, Zou XL, Zheng YL, Qiu FZ (2010). Changes of antioxidative enzymes and lipid peroxidation in leaves and roots of waterlogging-tolerant and waterlogging-sensitive maize genotypes at seedling stage. Agric Sci Chin 9, 651-661.
Vert GA, Briat J-F, Curie C (2003). Dual regulation of the Arabidopsis high-affinity root iron uptake system by local and long-distance signals. Plant Physiol 132, 796-804.
Wisniewski L, Dickinson NM (2003). Toxicity of copper to Quercus robur (English Oak) seedlings from a copper-rich soil. Environ Exp Bot 50, 99-107.
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[1] Lu Zhong-shu. Plant Growth Regutators in Relation to Plant Water Status[J]. Chin Bull Bot, 1985, 3(04): 1 -6 .
[2] Li Da Jue;Han Yun-zhou and Wan Li-ping. Studies on Germplasm Collections of Carthamus tinctorius IV Screening of the characterization of Seed Domancy[J]. Chin Bull Bot, 1990, 7(02): 50 -52 .
[3] . [J]. Chin Bull Bot, 1999, 16(增刊): 45 -46 .
[4] Yang Hong-yuan. Basic Principle and Method of Fluorescence Microscopy[J]. Chin Bull Bot, 1984, 2(06): 45 -48 .
[5] LU Jin-Yao;LUO Ai-Ling and LIANG Zheng. Some Improvement of TD-PAGE Technology[J]. Chin Bull Bot, 1998, 15(03): 69 -72 .
[6] LI Ling-Hao and CHEN Zuo-Zhong. The Global Carbon Cycle in Grassland Ecosystems and Its Responses to Global Change I . Carbon Flow Compartment Model, Inputs and Storage[J]. Chin Bull Bot, 1998, 15(02): 14 -22 .
[7] Huanhuan Xu, Jian Kang, Mingxiang Liang. Research Advances in the Metabolism of Fructan in Plant Stress Resistance[J]. Chin Bull Bot, 2014, 49(2): 209 -220 .
[8] . [J]. Chin Bull Bot, 2013, 48(1): 4 -5 .
[9] . [J]. Chin Bull Bot, 1996, 13(专辑): 45 .
[10] SHU Qun-Fang;ZHOU Lu;LI Wen-Bin;ZHANG LI-Ming and SUN Yong-Ru. Study on Gel Electrophoresis of Protein from Plant and Our Improved Methods[J]. Chin Bull Bot, 1998, 15(06): 73 -78 .