PDR 2015, 37(4) 428-438 DOI:    10.7677/ynzwyj201514058  ISSN: 2095-0845 CN: 53-1217/Q

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Keywords
Arabidopsis
Wounding
Membrane lipid
PLDδ
Phosphatidic acid
Authors
LI Ai-Hua-1、2
LING Li-Zhen-1
LI Wei-Qi-1
PubMed
Article by Li, A. H. 1、2
Article by Ling, L. Z. 1
Article by Li, W. Q. 1

Phospholipase Dδ is Involved in WoundingInduced Phosphatidic Acid Formation in Arabidopsis

 LI  Ai-Hua-1、2, LING  Li-Zhen-1, LI  Wei-Qi-1

1 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650201, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China

Abstract

Phosphalipase D (PLD) hydrolyzes phospholipids into phosphatidic acid (PA). PLDα1 and δ are the two most abundant members of the 12member PLD family in Arabidopsis. PLDα1 has been demonstrated having role in the woundinginduced PA signalling. However, whether and how PLDδ is involved in woundinginduced PA formation remained unclear. In the present study, the membrane lipids response to wounding was profiled in Wassilewskija (WS) and PLDδ knockout mutant (PLDδKO) of Arabidopsis. The levels of most lipids, including monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylcholine and phosphatidylglycerol had decreased rapidly within 30min after wounding in the two Arabidopsis genotypes. In contrast, the level of PA increased sharply and significantly 30min after wounding. It continued to increase until peaking at 1h postwounding in WS and 3h postwounding in PLDδKO, and then decreased. The PA levels were similar in the two genotypes in untreated leaves and in leaves 6h after wounding. However, these levels were lower in PLDδKO than in WS from 30min to 3h postwounding. The significant difference of PA level between the two genotypes occurred 30min after wounding, when it was about 20% lower in PLDδKO than in WS. These results show that PLDδ is involved in woundinginduced PA formation in Arabidopsis, but its absence induces PA response later and with less intensity than PLDα1.

Keywords Arabidopsis   Wounding   Membrane lipid   PLDδ   Phosphatidic acid  
Received 2014-04-06 Revised  Online: 2014-06-10 
DOI: 10.7677/ynzwyj201514058
Fund:

The National Science Foundation of China (NSFC31300261)

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