PDR 2015, 37(4) 453-462 DOI:   10.7677/ynzwyj201514151  ISSN: 2095-0845 CN: 53-1217/Q

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Castor bean
GATA gene family
Gene expression
Dark treatment
AO Tao-1、3
LIAO Xiao-Jia-2、3
XU Wei-2、3
LIU Ai-Zhong-1
Article by Ao, T. 1、3
Article by Liao, X. J. 2、3
Article by Xu, W. 2、3
Article by Liu, A. Z. 1

Identification and Characterization of GATA Gene Family in Castor Bean (Ricinus communis)

 AO  Tao-1、3, LIAO  Xiao-Jia-2、3, XU  Wei-2、3, LIU  Ai-Zhong-1

1 Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Science,
Mengla, Yunnan 666303, China; 2 Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201 China;
3 Graduate University of Chinese Academy of Science, Beijing 100049, China


GATA proteins are considered to be broadly involved in yieldassociated biological process, such as photoresponse, chlorophyll biosynthesis, and carbon and nitrogen metabolism. Based on castor bean genome database, a total of 19 GATA genes were identified and classified into 4 subfamilies according to gene structure, protein structure and their phylogenetic relationships. Results exhibited that GATA factors were hydrophilic proteins. Analysis of gene structure and protein structure revealed the conserved structural features of GATA factors between castor bean and Arabidopsis thaliana. The highthroughput RNAseq data were used to define the expressional profiles of GATA genes among tissues. The results showed that most of the castor GATA genes preferentially expressed in leaf and root in contrast to their expression in developing seeds. In particular, the expression of GATA genes responding to darkness treatment in leaves was detected using semiquantitative RTPCR. It was shown that expression of three genes was downregulated under darkness treatment, which suggests a role for GATA genes of castor bean in lightmediated regulation. These results provide important theoretical basis to the functions identification of castor GATA genes and increase castor yields.

Keywords Castor bean   GATA gene family   Gene expression   Dark treatment  
Received 2014-11-11 Revised  Online: 2015-03-27 
DOI: 10.7677/ynzwyj201514151

国家自然科学基金 (31401421)

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