Acta Metallurgica Sinica 2007, 29(04) 388-392 DOI:      ISSN: 0412-1961 CN: 21-1139/TG

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Keywords
Paris polyphylla
Authors
HE Jun1 , 2 , YANG Bai-Yun1 , CHEN Shao-Feng1 , GAO Lian-Ming2 , WANG Hong2
PubMed
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Assessment of Genetic Diversity of Paris polyphylla (Trilliaceae) by ISSR Markers

HE Jun1 , 2 , YANG Bai-Yun1 , CHEN Shao-Feng1 , GAO Lian-Ming2 , WANG Hong2

1 Colleage of Life Science , Nanchang university, Nanchang 330047 , China; 2 Laboratory of biodiversity and biogeography ,
Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204 , China

Abstract

Some species of the genus Paris are of high medicinal value . Severe human activities particularly over-harvesting have led to recent declines of their population size and fragmented habitat patches . Inter-simple sequence repeat markers ( ISSR) were applied to investigate the genetic diversity of eight populations of Paris polyphylla in Yunnan and Sichuan Provinces. Fourteen primers resulted to a total of 251 bands from the eight populations, of which 235 were polymorphic, which showes a high level of genetic diversity in this species . A relatively high level of genetic diversity was revealed: PPB
= 93 . 63% , HE = 0 . 2204 and HO = 0. 3532 at the species level; PPB = 50. 45%, HE = 0.1405 and HO = 0.2194 at the population level. The GST= 0. 3625 indicated a high degree of genetic differentiation among populations . Genetic variation occurred mainly within populations . In UPGMA cluster analysis the six populations of P. polyphylla var. yunnanensis were divided into two clades , and P. polyphylla var. yunnanensis is more related to P. polyphylla var . stenophylla than to P. polyphylla var . pseudothibetica . In this paper , some conservation concerns were discussed which would be helpful for the conservation and sustainable utilization of this medicine resources.

Keywords Paris polyphylla  
Received 2006-10-09 Revised 1900-01-01 Online:  
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Corresponding Authors: WANG Hong
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