Acta Metallurgica Sinica 2007, 29(04) 457-460 DOI:      ISSN: 0412-1961 CN: 21-1139/TG

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Keywords
Piper nigrum
Authors
SHI Jiang1 , XIN Li1 , TAN Lin2 , ZHENG Xue-Qin2
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Study on the RAPD Specific Band of Kava ( Piper , Piperaceae) Transferring to the SCAR Molecular Marker

SHI Jiang1 , XIN Li1 , TAN Lin2 , ZHENG Xue-Qin2

1 Agriculture College, Henan University of Science & Technology, Luoyang 471003 , China ; 2 State Key Biotechnology Laboratory for Tropical Crops , South China University of Tropical Agriculture , Haikou 571101 , China

Abstract

This paper studied on 28 pepper materials, including 16 cultivated papper materials, 3 wild pepper materials, 2 wild relatives of pepper materials , 6 kava ( Piper methysticum) materials, and 1 Peperomia pellucida materials. According to RAPD analysis, we generate SCAR marker for identifying Kava ( Piper, Piperaceae ) . A Kava-associated fragment with a length ofabout 400 bp was generated with OPQ-03 primer . The fragment was cloned and sequenced . PCR amplification with the specific primers P7 . 1 ( 5′-GGT CAC CTC ACC GCA GCA GGA TGA ACG-3′) and P7 .2 (5′-GGT CAC CTC AAT GAC ATG GGA TGA ATC-3′) was performed to 28 materials , which 27 materials amplified the 440 bp specific band except for Peperomia pellucida Kunth .

Keywords Piper nigrum  
Received 2006-10-27 Revised 1900-01-01 Online:  
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Corresponding Authors: SHI Jiang
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