Chinese Bulletin of Botany ›› 2007, Vol. 24 ›› Issue (04): 526-531.

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Application of Bioreactors in Rapid Propagation of Gypsophila paniculata

Chunhua Zhang Xuanchun Piao Meilan Lian Shouming Wang   

  1. Department of Horticulture, Agricultural College of Yanbian University, Longjing 133400, China
  • Received:2006-10-24 Revised:2006-12-25 Online:2007-07-01 Published:2007-07-01
  • Contact: Meilan Lian

Abstract: We investigated proliferation culture of Gypsophila paniculata by using a raft method in a 2.5 L air-lift bioreactor of the column type. Inoculation density of 35 explants in the bioreactor was more favorable than treatments of 25 and 45 explants for proliferation; 252 vigorous adventitious shoots were obtained for the 35 explants with the bioreactor. A light intensity of 90mmol·m-2·s-1 was considered the most effective, but excessive light inhibited shoot formation and length. Furthermore, proliferation of G. paniculata in the bioreactor was more efficient with lacunaris sparger of small pore size (15 mm) and 0.1 vvm of air.