Abstract: The genomic DNAs extracted from 1/2 mature seed of oilseed sunflower hybrid A15 and its parents were analyzed using AFLP (Amplified fragment length polymorphisms) to generate DNA fingerprints. A total of 1125 AFLP markers were produce with 17 primer combinations to screen A_line and R_line, ranging from 100～500 base pairs (bp), and the polymorphism rate was about 12.8%, the average bands amplified by each primer combination were 66. Primer combination E_AAC/M_CTC, could generate characteristic bands: 440 bp、190 bp、160 bp in A_line and 380 bp、350 bp、225 bp、180 bp in R_line, whereas E_ACG/M_CTG could generate characteristic bands: 480 bp, 265 bp in A_line and 490 bp、220 bp、205 bp and 125 bp in R_line. All these characteristic bands screened were found in A15 hybrids. These bands c an be used as AFLP markers to identify different varieties in oilseed sunflower and the purity of A15 hybrid seed. Several assess methods of identification on varieties and purity of hybrid were discussed in this paper.