Abstract: During strawberry （Fragaria ananassa Duch cv.chunxing）fruits maturation，the content of total sugar，anthocyanin and respiration rate increased significantly，and Ca2+-ATPase activity of cytoplasm and microsome membrane increased firstly，then peaked at pink stage，and decreased at full red stage in fruits，and also，the activity of plasma membrane Ca2+-ATPase was the highest in microsome membranes. Na3VO4，an inhibitor of plasma membrane Ca2+-ATPase activity，stimulated accumulation of anthocyanin，declined the total sugar content，but had different effects with various fruit mature degree.