Chinese Bulletin of Botany     2015 50 (5):  637-    ISSN: 1674-3466:  CN: 11-5705/Q Plant Microfluidic Chip, an Integrated High-throughput Platform for Real-time Analysis of Plant Growth and Development Minghong Wang1, Lai Ma2, Xiaojiang Zheng1, Yibing Hu1,2,3* 1Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, Enshi 445000, China 2College of Resources & Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China 3State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210076, China Received 2014-09-29  Revised null  Online 2015-09-01 Reference  [1]Bermejo C, Haerizadeh F, Takanaga H, Chermak D, and Frommer WB .Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.[J].Biochem J, 2010, 432 (2):399-406 [2]Bermejo C, Haerizadeh F, Takanaga H, Chermak D, and Frommer WB .Optical sensors for measuring dynamic changes in steady state levels of cytosolic glucose in yeast.[J]., 2011a, 6 :1806-1817 [3]Bermejo C, Ewald J, Lanquar V, Jones A, and Frommer WB.In vivo biochemistry: Quantifying ion and metabolite levels in individual cells or cultures of yeast. [J].Biochem J, 2011b, 438 :1-10 [4]Campbell RE .Fluorescent-protein-based biosensors: Modulation of energy transfer as a design principle. [J]., 2009, 81 (15):5972-5979 [5]Chaudhuri B, H?rmann F, Frommer WB .Dynamic imaging of glucose flux impedance using FRET sensors in wild-type Arabidopsis plants. [J]., 2011, 62 (7):2411-2417 [6]Davidson MW, Campbell RE.Engineered fluorescent proteins: innovations and applications.[J].Nat Meth, 2009, 6 (10):713-717 [7]Dean KM, Qin Y, Palmer A .Visualizing metal ions in cells: An overview of analytical techniques, approaches, and probes.[J].BBA - Molecular Cell Res, 2012, 1823 (0):1406-1415 [8]Denninger P, Bleckmann A, Lausser A, Vogler F, Ott T, Ehrhardt DW, Frommer WB, Sprunck S, Dresselhaus T, Grossmann G.Male-female communication triggers calcium signatures during fertilization in Arabidopsis. [J]., 2014, 5 (0):4645- [9]Ehrhardt DW, Frommer WB.New technologies for 21st century plant science.[J]., 2012, 24. (2):374-394 [10]Emmanouilidou E, Teschemacher AG, Pouli AE, Nicholls LI, Seward EP, Rutter GA .Imaging Ca2+ concentration changes at the secretory vesicle surface with a recombinant targeted cameleon. [J].Curr Bio l, 1999, 9 :915-918 [11]Grossmann G, Meier M, Cartwright HN, Sosso D, Quake SR, Ehrhardt DW, Frommer WB.Time-lapse fluorescence imaging of Arabidopsis root growth with rapid manipulation of the root environment using the Root Chip[J]., 2012, 65 : 4290- [12]Haydon MJ .Getting a sense for zinc in plants.[J]., 2014, 202 :10-12 [13]Horade M, Yanagisawa N, Mizuta Y, Higashiyama T, Arata H .Growth assay of individual pollen tubes arrayed by microchannel device.[J]., 2014, 118 :25-28 [14]Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, Frommer WB.Abscisic acid dynamics in roots detected with genetically encoded FRET sensors. [J]., 2014, :3:e01741- [15]Jones AM, Grossmann G, Danielson J?, Sosso D, Chen LQ, Ho CH, Frommer WB.In vivo biochemistry: applications for small molecule biosensors in plant biology. [J]., 2013, 16 :389-395 [16]Kao JPY, Li G, Auston DA .Chapter 5 - Practical Aspects of Measuring Intracellular Calcium Signals with Fluorescent Indicators.[J].Methods Cell Biol, 2010, 99 :113-152 [17]Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, Kudla J, Schumacher K.FRET-based genetically encoded sensors allow high-resolution live cell imaging of Ca2+ dynamics. [J]., 2012, 69 (1):181-192 [18]Lanquar V, Grossmann G, Vinkenborg JL, Merkx M, Thomine S, Frommer WB.Dynamic imaging of cytosolic zinc in Arabidopsis roots combining FRET sensors and RootChip technology. [J]., 2014, 202 :198-208 [19]Looger L，Lalonde S， Frommer WB.Genetically encoded FRET sensors for visualizing metabolites with subcellular resolution in living cells. [J].Plant Physiol, 2005, 138 :555-557 [20]Mank M, Santos AF, Direnberger S, Mrsic-Flogel TD, Hofer SB, Stein V, Hendel T, Reiff DF, Levelt C, Borst A, Bonhoeffer T, Hübener M, Griesbeck O .A genetically encoded calcium indicator for chronic in vivo two-photon imaging. [J]., 2008, 5 :805-811 [21]Mérola F, Fredj A, Betolngar DB, Ziegler C, Erard M, Pasquier H .Newly engineered cyan fluorescent proteins with enhanced performances for live cell FRET imaging.[J]., 2014, 9 :180 -191 [22]Meyer AJ, Dick TP .Fluorescent protein-based redox probes.[J]., 2000, 13 :621-650 [23]Okumoto S, Jones A, Frommer WB .Quantitative Imaging with Fluorescent Biosensors: Advanced Tools for Spatiotemporal Analysis of Biodynamics in Cells.[J].Annu Rev Plant Biol, 2012, 63 :26.1-.-26.43 [24]Okumoto S.Quantitative imaging approaches for small-molecule measurements using FRET sensors in plants. [J]., 2014, 1083 :55-64 [25]Park JG, Palmer AE. Quantitative measurement of Ca2+ and Zn2+ in mammalian cells using genetically encoded fluorescent biosensors[J]., 2014, 1071 :29　 -47 [26]Nezhad SA.Microfluidic platforms for plant cells studies.[J]., 2014, 14 :3262-3274 [27]Nezhad SA, Naghavi M, Packirisamy M, Bhat R, Geitmann A .Quantification of cellular penetrative forces using lab-on-a-chip technology and finite element modeling.[J]., 2013, 110 :8093-8098 [28]Nezhad SA, Packirisamy M, Bhat R, Geitmann A .In vitro study of oscillatory growth dynamics of Camellia pollen tubes in microfluidic environment.[J]., 2013, ( 60):3185-3193 [29]Sozzani R Busch, Spalding EP, Benfey PN .Advanced imaging techniques for the study of plant growth and development.[J]., 2014, 19 :304-310 [30]Unger MA, Chou HP, Thorsen T, Scherer A, and Quake SR.Monolithic microfabricated valves and pumps by multilayer soft lithography. [J].Science, 2000, 288 :113-116 [31]Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M .Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis. [J]., 2009, 6 :737-740 [32]Whitesides GM.The origins and the future of microfluidics.[J].Nature, 2006, (442):368-373 [33]Yetisen AK, Jiang L, Cooper JR, Qin Y, Palanivelu R and Zohar Y.A microsystem-based assay for studying pollen tube guidance in plant reproduction.[J].Micromech Microeng, 2011, 21 :054018- Corresponding author: Hu Yibing