Phylogenetic estimation and morphological evolution of Alsineae (Caryophyllaceae) shed new insight into the taxonomic status of the genus Pseudocerastium

doi:10.1016/j.pld.2020.11.001

Plant Diversity ›› 2021, Vol. 43 ›› Issue (04): 299-307.DOI: 10.1016/j.pld.2020.11.001

Phylogenetic estimation and morphological evolution of Alsineae (Caryophyllaceae) shed new insight into the taxonomic status of the genus Pseudocerastium

Gang Yao^a, Bine Xue^b, Kun Liu^c, Yuling Li^a, Jiuxiang Huang^a, Junwen Zhai^d

a College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China;
b College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China;
c College of Life Sciences, Anhui Normal University, Wuhu, 241000, Anhui, China;
d College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

收稿日期:2020-06-01 修回日期:2020-10-29 出版日期:2021-08-25 发布日期:2021-09-07
通讯作者: Junwen Zhai
基金资助:
The authors are indebted to Dr. Xinxin Zhu from Xinyang Normal University, China, for providing field images of Cerastium jiuhuashanense and C. wilsonii, for Dr. Jacob B. Landis from Cornell University, USA, for revising the English writing. This study was financially supported by grant awards from the Natural Science Foundation of Guangdong Province, China (2019A1515011695) and the National Natural Science Foundation of China (31500180).

Phylogenetic estimation and morphological evolution of Alsineae (Caryophyllaceae) shed new insight into the taxonomic status of the genus Pseudocerastium

Gang Yao^a, Bine Xue^b, Kun Liu^c, Yuling Li^a, Jiuxiang Huang^a, Junwen Zhai^d

a College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China;
b College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China;
c College of Life Sciences, Anhui Normal University, Wuhu, 241000, Anhui, China;
d College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

Received:2020-06-01 Revised:2020-10-29 Online:2021-08-25 Published:2021-09-07
Contact: Junwen Zhai
Supported by:
The authors are indebted to Dr. Xinxin Zhu from Xinyang Normal University, China, for providing field images of Cerastium jiuhuashanense and C. wilsonii, for Dr. Jacob B. Landis from Cornell University, USA, for revising the English writing. This study was financially supported by grant awards from the Natural Science Foundation of Guangdong Province, China (2019A1515011695) and the National Natural Science Foundation of China (31500180).

摘要/Abstract

摘要： Pseudocerastium is a monotypic genus in Caryophyllaceae endemic to China. The genus has been widely accepted since it was described in 1998, however its phylogenetic position within Caryophyllaceae has never been studied. In the present study, the whole plastid genome and nuclear ribosomal internal transcribed spacer (ITS) sequences of Pseudocerastium stellarioides was obtained through genome skimming, and the phylogenetic position of the species was studied for the first time. Plastid phylogenomic analysis of Caryophyllaceae revealed that Pseudocerastium is clustered within the tribe Alsineae with strong support. Phylogenetic analyses based on an enlarged taxon sampling of Alsineae using five DNA regions (matK, rbcL, rps16 intron, trnL-F and ITS) revealed that P. stellarioides was nested deeply within Cerastium with strong support. Analyses of morphological character evolution suggest that the ancestral states in Alsineae include three styles and a six-lobed capsule at the apex, while both Cerastium and Pseudocerastium have five styles and ten lobes at the apex of the capsule, further supporting their close relationship. The species Pseudocerastium stellarioides is similar to Cerastium wilsonii in morphology, but differs in having villous indumentum on the lower part of the filaments and compressed globose seeds. Therefore, based on the present molecular and morphological evidence, the generic name Pseudocerastium is reduced here as a new synonym of Cerastium and the species P. stellarioides is transferred to Cerastium as C. jiuhuashanense.

关键词: Alsineae, Cerastium, Character evolution, Genome skimming, Molecular phylogeny, Taxonomy

Abstract: Pseudocerastium is a monotypic genus in Caryophyllaceae endemic to China. The genus has been widely accepted since it was described in 1998, however its phylogenetic position within Caryophyllaceae has never been studied. In the present study, the whole plastid genome and nuclear ribosomal internal transcribed spacer (ITS) sequences of Pseudocerastium stellarioides was obtained through genome skimming, and the phylogenetic position of the species was studied for the first time. Plastid phylogenomic analysis of Caryophyllaceae revealed that Pseudocerastium is clustered within the tribe Alsineae with strong support. Phylogenetic analyses based on an enlarged taxon sampling of Alsineae using five DNA regions (matK, rbcL, rps16 intron, trnL-F and ITS) revealed that P. stellarioides was nested deeply within Cerastium with strong support. Analyses of morphological character evolution suggest that the ancestral states in Alsineae include three styles and a six-lobed capsule at the apex, while both Cerastium and Pseudocerastium have five styles and ten lobes at the apex of the capsule, further supporting their close relationship. The species Pseudocerastium stellarioides is similar to Cerastium wilsonii in morphology, but differs in having villous indumentum on the lower part of the filaments and compressed globose seeds. Therefore, based on the present molecular and morphological evidence, the generic name Pseudocerastium is reduced here as a new synonym of Cerastium and the species P. stellarioides is transferred to Cerastium as C. jiuhuashanense.

Key words: Alsineae, Cerastium, Character evolution, Genome skimming, Molecular phylogeny, Taxonomy

Gang Yao, Bine Xue, Kun Liu, Yuling Li, Jiuxiang Huang, Junwen Zhai. Phylogenetic estimation and morphological evolution of Alsineae (Caryophyllaceae) shed new insight into the taxonomic status of the genus Pseudocerastium[J]. Plant Diversity, 2021, 43(04): 299-307.

参考文献

Dillenberger, M.S., Kadereit, J.W., 2014. Maximum polyphyly:Multiple origins and delimitation with plesiomorphic characters require a new circumscription of Minuartia (Caryophyllaceae). Taxon 63, 64-88.
Greenberg, A.K., Donoghue, M.J., 2011. Molecular systematics and character evolution in Caryophyllaceae. Taxon 60, 1637-1652.
Harbaugh, D.T., Nepokroeff, M., Rabeler, R.K., et al., 2010. A new lineage-based tribal classification of the family Caryophyllaceae. Int. J. Plant Sci. 171, 185-198.
Hernandez-Ledesma, P., Berendsohn, W.G., Borsch, T., et al., 2015. A taxonomic backbone for the global synthesis of species diversity in the angiosperm order Caryophyllales. Willdenowia 45, 281-383.
Jin, J.J., Yu, W.B., Yang, J.B., et al., 2020. GetOrganelle:a fast and versatile toolkit for accurate de novo assembly of organelle genomes. Genome Biol. 21, 241. https://doi.org/10.1186/s13059-020-02154-5
Ke, P., 1996. Cerastium. In:Tang CL (ed.) Flora Reipublicae Popularis Sinicae 26. Science Press, Beijing, 76-93.
Li, Y.L., Huang, J.X., Yao, G., 2019. Characterization of the complete plastid genome of Psammosilene tunicoides (Caryophyllaceae), and endangered medical herb endemic to south-western China. Mitochondrial DNA B. 4, 2798-2799.
Lu, D.Q., Wu, Z.Y., Zhou, L.H., et al., 2001. Caryophyllaceae. In:Wu ZY, Raven PH (eds) Flora of China 6. Science Press & Missouri Botanical Garden Press, Beijing & St. Louis, 1-113.
Maddison, W.P., Maddison, D.R., 2014. Mesquite:Amodular System for Evolutionary Analysis. Version 3.01. http://mesquiteproject.org.
Miller, M.A., Pfeiffer, W., Schwartz, T., 2010. Creating the CIPRES Science Gateway for inference of large phylogenetics trees. In:Proceedings of the Gateway Computing Environments Workshop (GCE). New Orleans, LA, 1-8.
Posada, D., 2008. JModelTest:phylogenetic model averaging. Mol. Biol. Evol. 25:1253-1256.
Pusalkar, P.K., Singh, D.K., 2015. Taxonomic rearrangement of Arenaria (Caryophyllaceae) in Indian Western Himalaya. J. Jpn. Bot. 90, 77-91.
Qu, X.J., Moore, M.J., Li, D.Z., et al., 2019. PGA:a software package for rapid, accurate, and flexible batch annotation of plastomes. Plant Methods 15, 50.
Rambaut, A., Suchard, M.A., Drummond, A.J., 2014. Tracer v1.6. Available at:http://beast.bio.ed.ac.uk/Tracer.
Ronquist, F., Huelsenbeck, J.P., 2003. MrBayes 3:Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574.
Sadeghian, S., Zarre, S., Rabeler, R.K., et al., 2015. Molecular phylogenetic analysis of Arenaria (Caryophyllaceae:tribe Arenarieae) and its allies inferred from nuclear DNA internal transcribed spacer and plastid DNA rps16 sequences. Bot. J. Linn. Soc. 178, 648-669.
Sharples, M.T., Tripp, E.A., 2019. Phylogenetic relationships within and delimitation of the cosmopolitan flowering plant genus Stellaria L. (Caryophyllaceae):core stars and fallen stars. Syst. Bot. 44, 857-876.
Stamatakis, A., 2006. RAxML-VI-HPC:maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22, 2688-2690.
Tian, X., Luo, J., Wang, A., et al., 2011. On the origin of the woody buckwheat Fagopyrum tibeticum (=Parapteropyrum tibeticum) in the Qinghai-Tibetan Plateau. Mol. Phylogent. Evol. 61, 515-520.
Wang, L.S., Jia, Y., Zhang, X.C., Qin, H.N., 2015. Overview of higher plant diversity in China. Biodiv. Sci. 23, 217-224.
Wu, Z.Y., Sun, H., Zhou, Z.K., et al., 2005. Origin and differentiation of endemism in the flora of China. Acta Bot. Yunnan. 27, 577-604.
Xie P, Tu T, Razafimandimbison SG, et al., 2014. Phylogenetic position of Guihaiothamnus (Rabiaceae):Its evolutionary and ecological implications. Mol. Phylogent. Evol. 78, 375-385.
Xu, B., Luo, D., Li, Z.M., et al., 2019. Evolutionary radiations of cushion plants on the Qinghai-Tibet Plateau:Insights from molecular phylogenetic analysis of two subgenera of Arenaria and Thylacospermum (Caryophyllaceae). Taxon 68, 1003-1020.
Yao, G., Jin, J.J., Li, H.T., et al., 2019. Plastid phylogenomic insights into the evolution of Caryophyllales. Mol. Phylogent. Evol. 134, 74-86.
Yang, B., Li, L., Liu, J., et al., 2020. Plastome and phylogenetic relationship of the woody buckwheat Fagopyrum tibeticum in the Qinghai-Tibet Plateau. Plant Divers. https://doi.org/10.1016/j.pld.2020.10.001
Yu, S.X., Hao, G., Jin, X.F., 2016. Caryophyllaceae. In:Species Catalogue of China Vol. 1 Plants, Spermatophytes (VII), Angiosperms (Caryophyllaceae-Ericaceae). Science Press, Beijing, 1-33.
Zeng, C.X., Hollingsworth, P.M., Yang, J., et al., 2018. Genome skimming herbarium specimens for DNA barcoding and phylogenomics. Plant Methods 14, 43.
Zhang, M.L., Zeng, X.Q., Li, C., et al., 2017. Molecular phylogenetic analysis and character evolution in Pseudostellaria (Caryophyllaceae) and description of a new genus, Hartmaniella, in North America. Bot. J. Linn. Soc. 184, 444-456.
Zhang, X.P., Guo, X.H., 1998. A new genus of Caryophyllaceae from China. Acta Bot. Yunnan. 20, 395-398.

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Phylogenetic estimation and morphological evolution of Alsineae (Caryophyllaceae) shed new insight into the taxonomic status of the genus Pseudocerastium

Phylogenetic estimation and morphological evolution of Alsineae (Caryophyllaceae) shed new insight into the taxonomic status of the genus Pseudocerastium

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