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Plant Diversity ›› 2009, Vol. 31 ›› Issue (03): 247-252.DOI: 10.3724 SP.J.1143.2009.09019

• 研究论文 • 上一篇    下一篇


宋 莉1 , 2 , 赵德刚1 , 3, 吴拥军3   

  1. 1 贵州省农业生物工程重点实验室, 贵州贵阳 550025; 2 教育部绿色农药与农业生物工程重点实验室, 贵州贵阳 550025; 3 贵州大学生命科学学院, 贵州贵阳 550025
  • 收稿日期:2009-02-04 出版日期:2009-06-25 发布日期:2009-06-25
  • 通讯作者: 赵德刚

Expression of Bioactive Chicken Alpha Interferon in Transgenic Tobacco

SONG Li1 , 2 , ZHAO De-Gang1 , 3 , WU Yong-Jun3   

  1. 1 Guizhou Key Laboratory of Agricultural Bioengineering , Guiyang 550025 , China; 2 Key Laboratory of Green Pesticide and Agricultural Biological Engineering, Ministry of Education , Guiyang 550025 , China; 3 College of Life Sciences, Guizhou University , Guiyang 550025 , China
  • Received:2009-02-04 Online:2009-06-25 Published:2009-06-25

摘要: 利用植物生物反应器生产外源药用蛋白近年来备受关注, 本研究通过农杆菌介导法, 将人工合成的鸡α干扰素基因( ChIFN-α) 转化烟草( Nicotiana tabacum) 无菌苗叶盘。对抗性植株进行的GUS 活性鉴定,PCR 和RT-PCR 检测表明, ChIFN-α基因已整合到烟草基因组中并具有转录活性, ELISA 检测和细胞病变(CPE) 抑制试验表明转基因烟草表达的干扰素蛋白具有抗病毒活性。

关键词: 鸡α干扰素, 烟草, 表达, 生物学活性

Abstract: Expression of avian cytokine in tobacco or other plants had the economically potential use for producing pharmacological applications products and applying easily in practice . The cDNA encoding chicken alpha interferon (ChIFN-α) was introduced into Nicotiana tabacum Xanthin by Agrobacterium transformation . The transgenic plants were detected by histochemical stain assay and molecular analysis . Recombinant ChIFN-αwas confirmed by enzyme- linked immunosorbent assay (ELISA) and cytopathic effect (CPE) inhibition assay . The results showed that ChIFN-α gene was successfully introduced into tobacco plants and stably expressed at various level from 0 .0003% to 0. 0033% of total soluble protein. The biological activity of ChIFN-αwas 5 .01×103 IU􊄯g of tissue in vitro . Since interferon was involved in host antiviral response, our results provided the possibility for further study of plant oral adjuvant with ChIFN-αfor improving immunity in birds .

Key words: ChIFN-α