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Plant Diversity ›› 2009, Vol. 31 ›› Issue (04): 303-308.DOI: 10.3724 SP.J.1143.2009.09035

• 研究论文 • 上一篇    下一篇

烟草雌、雄配子DNA 含量变化

林美珍1 , 邓 桦2 , 魏冬梅2 , 田惠桥2   

  1. 1 漳州卫生职业学院药学系, 福建漳州 363000 ; 2 厦门大学生命科学学院, 福建厦门 361005
  • 收稿日期:2009-02-24 出版日期:2009-08-25 发布日期:2009-08-25
  • 通讯作者: 田惠桥

Change of DNA Content in Male and Female Gametes of Tobacco ( Nicotiana tabacum)

LIN Mei-Zhen1 , DENG Hua2 , WEI Dong-Mei2 , TIAN Hui-Qiao2   

  1. 1 Department of Pharmacy , Zhangzhou Medical Vocational College, Zhangzhou 363000 , China;
    2 School of Life Sciences , Xiamen University , Xiamen 361005 , China
  • Received:2009-02-24 Online:2009-08-25 Published:2009-08-25
  • Contact: TIAN Hui-Qiao

摘要: 采用显微分光光度法测定了烟草( Nicotiana tabacum) 精细胞和卵细胞的DNA 含量。烟草是二胞花粉, 花粉萌发后生殖细胞在花粉管中分裂形成精细胞。授粉后45 h 花粉管到达子房, 在花粉管内的精细胞DNA 含量为1C。当花粉管在退化助细胞中破裂, 释放出的两个精细胞开始合成DNA。在与卵细胞融合前,两个精细胞DNA 含量接近2C。随着精细胞的到达及合成DNA, 卵细胞也开始合成DNA, 融合前的卵细胞DNA 含量也接近2C。精、卵细胞融合后, 合子DNA 含量为4C。烟草雌、雄配子是在细胞周期的G2 期发生融合, 属于G2 型。

关键词: 烟草, 精细胞, 卵细胞, DNA 含量

Abstract: The nuclear DNA content of male and female gametes of tobacco ( Nicotiana tabacum) was measured using DAPI stain and microspectrofluorimetric measurement. Tobacco pollen is bicellular at anthesis, containing a vegetative cell and a generative cell , which divides to form two sperm cells in a pollen tube. The nuclear DNA content of generative cell in a pollen tube was at 2C level and that of two sperm cells in a pollen tube, which elongated in the style, at 1C level. Two sperm cells began to synthesize DNA after both were released in the degenerated synergid , and the quantity of nuclear DNA
in both sperm cells approached 2C level before both fusing with egg and central cells . During this process , the nuclear DNA content of egg cell also began to increase and approached 2C level before fusing with sperm cell . After male and female gamete fused , the nuclear DNA content of zygote reached 4C level . Therefore , the fusion of male and female gametes of tobacco was at G2 of cell cycle, and this fusion of fertilization belonged to G2 type . This result displayed the fertilization multiformity in angiosperms .

Key words: Tobacco