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Plant Diversity ›› 2009, Vol. 31 ›› Issue (05): 415-420.DOI: 10.3724 SP.J.1143.2009.09175

• 研究论文 • 上一篇    下一篇

叶绿体分裂相关蛋白CrMinD 的保守功能

雷启义, 周江菊, 张文华   

  1. 凯里学院环境与生命科学学院, 贵州凯里 556000
  • 收稿日期:2009-09-07 出版日期:2009-10-25 发布日期:2009-10-25
  • 通讯作者: 雷启义

The Conservative Function of Chloroplast Division Associated CrMinD Protein

LEI Qi-Yi , ZHOU Jiang-Ju , ZHANG Wen-Hua   

  1. College of Life and Environment Science, Kaili University , Kaili 556000 , China
  • Received:2009-09-07 Online:2009-10-25 Published:2009-10-25
  • Contact: LEI Qi-Yi

摘要: 细胞或质体中部正确分裂位点的选择是MinD 蛋白与其他Min 蛋白(MinC􊄯E ) 相互作用的结果, MinD 蛋白在原核细胞以及植物叶绿体的分裂过程中发挥着重要的作用。细胞中MinD 蛋白浓度的明显升高可影响正常细胞的分裂过程而产生丝状体细胞。为了研究叶绿体分裂蛋白CrMinD 的保守功能, 构建了衣藻CrMinD-gfp 的原核表达重组质粒进行了原核功能验证。试验结果表明, 衣藻CrMinD 蛋白的过量表达严重影响了大肠杆菌的分裂, 其在原核细胞中运动和定位与用GFP 标记的原核细胞MinD 蛋白具有相似性。更进一步证明了叶绿体分裂同源物CrMinD 蛋白与原核细胞MinD 蛋白有着相似的功能, 是一个进化上功能保守的蛋白。同时, 这一结果也为研究植物细胞中质体的分裂机制奠定了一定的基础。

关键词: 叶绿体, MinD 蛋白, 基因表达, 细胞分裂

Abstract: The interacting of MinD and MinC and MinE proteins of Escherichia coli are required for proper placement of the division septum at midcell, The MinD protein plays a key role in the course of bacterial cell and plant chloroplast division. The over production of MinD protein can block the normal cell division and lead to a filamentation cell . In plant cells, There are clear similarities between bacterial and plastid division, but limited information exists regarding the mechanism of plastid division in higher plants . Here we constructed a expression plasmid encoding the full-length CrMinD, an
Chlamydomonas reinhardtii homologue of the bacterial MinD . In agreement with cell division studies in bacteria over expression of CrMinD in E. coli results in filamentation cell formation . The sesuggestion that CrMinD is an evolutionary conserved cell division protein .Meanwhile , the result lays a certain foundation for studying on the division mechanism of plant chloroplast.

Key words: Chloroplast