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Plant Diversity ›› 2009, Vol. 31 ›› Issue (04): 289-295.DOI: 10.3724 SP.J.1143.2009.09075

• 研究论文 •    下一篇

东亚———北美东部间断透骨草属的扩增片段长度多态性

聂泽龙1 , 2 , 文 军1 , 3 , 4 , 孙 航1   

  1. 1 中国科学院昆明植物研究所生物多样性与生物地理重点实验室, 云南昆明 650204; 2 中国科学院研究生院, 北京 100049; 3 美国史密森研究院国家自然历史博物馆植物系, 华盛顿 20013-7012; 4 中国科学院植物研究所系统与进化植物学重点实验室, 北京 100093
  • 收稿日期:2009-04-16 出版日期:2009-08-25 发布日期:2009-08-25
  • 通讯作者: 孙 航

AFLP Analysis of Phryma (Phrymaceae) Disjunct between Eastern Asia and Eastern North America

NIE Ze-Long1 , 2 , WEN Jun1 , 3 , 4 , SUN Hang1   

  1. 1 Key Laboratory of Biodiversity and Biogeography , Kunming Institute of Botany , Chinese Academy of Sciences , Kunming 650204 , China ;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049 , China ; 3 Department of Botany, National Museum of Natural History , MRC 166 , Smithsonian Institution , Washington , DC 20013-7012 , USA; 4 Key Laboratory of Systematic and Evolutionary Botany , Institute of Botany, Chinese Academy of Sciences, Beijing 100093 , China

  • Received:2009-04-16 Online:2009-08-25 Published:2009-08-25
  • Contact: SUN Hang

摘要: 透骨草属( Phryma) 是一个单种属, 间断分布于东亚与北美东部。尽管东亚与北美东部居群形态差异非常小, 但分子变异却非常明显。本研究进一步运用AFLP 两对引物来衡量透骨草属的遗传多样性并评估其形态保守性。结果发现透骨草的遗传差异主要存在于两大洲的居群之间。聚类与PCA 分析显示透骨草分成两大支与其地理分布相吻合, 一支全部来自东亚, 另一支则是北美东部的居群。我们的结果强烈支持透骨草东亚———北美东部居群存在明显的遗传分化和形态保守。

关键词:

AFLP, 洲际间断, 东亚, 北美东部, 遗传多样性, 透骨草, 透骨草科

Abstract: Although little morphological differentiation is detected between intercontinental disjunct populations of Phryma, this monotypic genus shows distinct molecular divergence corresponding to its distribution in eastern Asia and eastern North America. This study further employs amplified fragment length polymorphism (AFLP) analyses using two selective primer pairs to quantify genetic diversity and evaluate the morphological stasis . Most of the molecular variance is accounted for by variance among populations between regions .Cluster and PCA analyses revealed that Phryma constitutes twomajor groups in line with their geographic distribution, with one genetically distinct group from the eastern Asia and the other with accessions from eastern North America. The results robustly supported the distinct genetic divergence and morphological stasis in Phryma.

Key words: AFLP

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