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Plant Diversity ›› 2009, Vol. 31 ›› Issue (05): 421-426.DOI: 10.3724/SP.J.1143.2009.09044

• 研究论文 • 上一篇    下一篇

高表达拟南芥miR396 提高烟草抗旱性

杨凤玺1 , 2 , 余迪求1   

  1. 1 中国科学院西双版纳热带植物园, 云南昆明 650223 ; 2 中国科学院研究生院, 北京 100049
  • 收稿日期:2009-03-09 出版日期:2009-10-25 发布日期:2009-10-25
  • 通讯作者: 余迪求

Overexpression of Arabidopsis MiR396 Enhances Drought Tolerance in Transgenic Tobacco Plants

YANG Feng-Xi1 , 2 , YU Di-Qiu1   

  1. 1 Xishuangbanna Tropical Botanical Garden , Chinese Academy of Sciences, Kunming 650223 , China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049 , China
  • Received:2009-03-09 Online:2009-10-25 Published:2009-10-25
  • Contact: YU Di-Qiu

摘要: MiR396 是一个由21 个核苷酸组成的单链非编码RNA 小分子。烟草内的miR396 受干旱诱导说明其可能参与烟草的干旱应答。在35S 强启动子作用下我们将miR396 转入到烟草体内获得高表达转基因植株, 生理学测试表明高表达miR396 的转基因烟草耐旱性增强, 同时叶片表现出比野生型较低的失水率和较高的相对含水量, 进一步分析表明转基因植株除了叶片变得更为窄小外, 其气孔密度和气孔系数都比野生型降低, 这些都表明miR396 作为一个正调节因子参与烟草的干旱胁迫应答。

关键词: miR396, 烟草, 干旱, 气孔

Abstract: MiR396 was a single-stranded noncoding small RNA with 21 nucleotides, and the expression of MiR369 in leaves was strongly induced in water deficit condition in tobacco , which suggested a possible role of miR396 in drought stress response. Under the control of 35S promoter, MIR396 was introduced into tobaccomediated by Agrobacterium tumefaciens. Physiological tests indicated that the elevated levels of miR396 increased drought tolerance in tobacco accompanying with lower water loss rate and higher relative water content. Further more miR396-overexpressing plants exhibited visible reductions both in stomatal density and stomatal index as well as a narrow and small leaf phenotype in comparison with wild- type plants. The present study indicated that miR396 was a positive regulator in response to drought stress in tobacco .

Key words: miR396