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Plant Diversity ›› 2010, Vol. 32 ›› Issue (4): 355-360.DOI: 10.3724/SP.J.1143.2010.09250

• 研究论文 • 上一篇    下一篇


 张利平1,2, , 余迪求1   

  1. 1 中国科学院西双版纳热带植物园, 云南 昆明650223; 2 中国科学院研究生院, 北京100049
  • 收稿日期:2009-12-22 出版日期:2010-08-25 发布日期:2010-02-04

Mutation of APETALA1 Affects the Basal Expression of WRKY Genes

 ZHANG  Li-Ping-1,2,  , YU  Di-Qiu-1   

  1. 1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2009-12-22 Online:2010-08-25 Published:2010-02-04


拟南芥APETALA1(AP1 )既是一个花分生组织特征基因又是一个花器官特征基因,在花器官发育中控制花萼和花瓣的发育。通过GUS染色进一步证实AP1主要在茎尖、花萼、花瓣和花托的位置表达。启动子分析发现,AP1启动子区包含了包括Wbox在内的大量顺式作用元件,暗示相关转录调控因子参与了对AP1的调控。21个WRKY基因单突变后并不改变AP1在花中的表达,但是AP1突变则增强了检测的10个WRKY基因中7个WRKY基因的表达,暗示AP1参与了对WRKY基因的基础表达的调控。这个结果也暗示AP1可能通过控制花萼和花瓣的发育从而参与了对花的基础抗性。

关键词: AP1, Wbox, WRKY, 基础表达, 基础抗性


The floral homeotic gene APETALA1 (AP1) specifies floral meristem identity and sepal and petal identity in Arabidopsis. GUS staining showed that the expression of AP1 mainly focus on shoot apex, sepal and petal, floral meristem, junction of siliques and pedicels. Sequence analysis showed that numerous ciselements were found in the AP1 promoter, including the Wbox, which indicated the regulated expression of AP1 by certain transcriptional factors. 21 independent wrky single mutants did not affect the expression of AP1 in inflorescence, however, mutation of AP1 enhanced the basal expression of seven WRKY genes among the 10 genes checked. This result implied that AP1 may participate in the basal resistance of flower through controlling the development of both sepals and petals.

Key words: AP1, Wbox, WRKY, Basal expression, Basal resistance