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Plant Diversity ›› 2011, Vol. 33 ›› Issue (3): 311-329.DOI: 10.3724/SP.J.1143.2011.10191

• 研究论文 • 上一篇    下一篇



  1. 中国科学院西双版纳热带植物园,云南 勐腊666303
  • 收稿日期:2010-11-02 出版日期:2011-06-25 发布日期:2011-02-10
  • 基金资助:


An Introduction to Cryopreservation of Plant Germplasm

 WEN  Bin   

  1. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China
  • Received:2010-11-02 Online:2011-06-25 Published:2011-02-10



关键词: 种质资源, 超低温保存, 超低温耐性, 超低温伤害, 顽拗性种子


This paper recited the history of plant germplasm cryopreservation, exhibited the diversity of cryopreservated plant materials, analysed biological foundation of plant cryotolerance, causes and types of cryo-injury, described the key techniques of frequently-used cryopreservation methods, and paid special attention to cryopreservation of plant germplasm of species producing recalcitrant seeds. Based on analysis of potential, status and difficulties, the author thought that regard to cryopreservation of plant germplasm of recalcitrant-seeded species there are much to do, for it was the keystone and difficult part of plant germplasm cryopreservation.

Key words: Germplasm, Cryopreservation, Cryotolerance, Cryo-injury, Recalcitrant seeds