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Plant Diversity ›› 2011, Vol. 33 ›› Issue (01): 91-100.DOI: 10.3724/SP.J.1143.2011.10247

• 研究论文 • 上一篇    下一篇


 宋敏舒1、2, 乐霁培2, 孙航2, 李志敏1   

  1. 1 云南师范大学生命科学学院,云南 昆明650092;2 中国科学院昆明植物研究所生物多样性与生物地理学重点实验室,云南 昆明650204
  • 收稿日期:2010-12-23 出版日期:2011-01-21 发布日期:2011-01-07
  • 基金资助:


Phylogeographical Study on Primula poissonii (Primulaceae) from Hengduan Mountains

 SONG  Min-Shu-1、2, LE  Ji-Pei-2, SUN  Hang-2, LI  Zhi-Min-1   

  1. 1 School of Life Science, Yunnan Normal University, Kunming 650092, China; 2 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China
  • Received:2010-12-23 Online:2011-01-21 Published:2011-01-07


由于横断山地区特殊的地理效应,第四纪冰期气候的反复变化对该地区植物的地理分布和居群遗传结构产生重要影响。为了揭示该地区分布的物种的谱系地理结构,检测了该地区特有的、生于湿地环境的植物海仙报春(Primula poissonii)的叶绿体trnL-F和trnT-L两个序列变异。共对13个居群167个个体的两个叶绿体片段进行测序,共发现11个单倍型。三种单倍型被共享,其他单倍型都只存在于单个居群内。分子变异分析 (AMOVA) 结果表明分布区内海仙报春的遗传变异主要存在于居群间 (99.08%), 且居群间的遗传分化很高 (GST =0.916, FST=0.99077, NST=0.973), 有着极显著的谱系地理学结构 (NST>GST, P<0.01) 和较低的居群间平均基因流 (Nm=0.08)。结合嵌套分支分析 (NCA),本文的研究结果推测该物种在冰期可能存在多个避难所, 并没有表现出大多数温带植物在第四纪冰期所经历过的种群集体扩张和迁移的现象,异域片段化被认为是该物种现有单倍型分布格局形成的主要原因。海仙报春的谱系地理结构是气候的反复波动和横断山特殊的地理环境互相作用的结果。

关键词: 横断山, 海仙报春, 谱系地理学, 叶绿体DNA, trnL-F, trnT-L


The topological effect of the Hengduan Mountains (HM) during the Quaternary climatic oscillation must have important effects on spatial distribution and genetic structure of plants distributed there. In this study, we examined the chloroplast trnL-F and trnT-L sequence variation of Primula poissonii, a relatively common alpine perennial endemic to this region. Eleven haplotypes were detected by analyzing 167 individuals from 13 populations of this species. Only three of them are commonly shared while the others are respectively fixed in the single population. An analysis of molecular variance (AMOVA) for populations of P.poissonii showed that the genetic variation mainly resided among populations (99.08%), and the estimates of interpopulation differentiation were very high (GST =0.916, FST=0.99077, NST=0.973). Further, a highly significant phylogeographic structure was present (NST>GST, P<0.01) whereas the value of average gene flow was low (Nm=0.08) in the entire geographical range. Along with the nested clade analysis (NCA), our results indicate there were multiple refugia for this species during the glacial stages. We failed to detect the interglacial or postglacial range expansion in this species as commonly revealed for the other temperate plants. The nested clade analysis indicated that allopatric fragmentation was likely the major processes that shaped the present spatial distribution of haplotypes in this species. The special phylogeographic structure of P.poissonii may have resulted from a combination of both climatic oscillation and complex topology of HM.

Key words: Hengduan Mountains, Primula poissonii, Phylogeography, Chloroplast DNA, trnL-F, trnT-L