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Plant Diversity ›› 2011, Vol. 33 ›› Issue (6): 674-682.DOI: 10.3724/SP.J.1143.2011.11060

• 研究论文 • 上一篇    下一篇

蒟蒻薯属 (薯蓣科) 植物DNA条形码研究

 赵月梅1、2, 张玲1   

  1. 1 中国科学院西双版纳热带植物园,云南 勐腊666303;2 中国科学院研究生院, 北京100049
  • 收稿日期:2011-04-11 出版日期:2011-12-25 发布日期:2011-07-05
  • 基金资助:

    中国科学院知识创新工程重要方向项目 (KSCX2-YW-Z-0904) 和中国科学院大科学装置工程项目 (2009-LSF-GBOWS-01)

Using DNA Barcoding in Genus Tacca (Dioscoreaceae)

 DIAO  Yue-Mei-1、2, ZHANG  Ling-1   

  1. 1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-04-11 Online:2011-12-25 Published:2011-07-05

摘要:

蒟蒻薯属(Tacca)植物种间在形态上差别不大,导致分类上存在一定的困难。DNA条形码是一种利用短的DNA标准片段来鉴别和发现物种的方法。本研究利用核基因ITS片段和叶绿体基因trnHpsbA, rbcL, matK片段对蒟蒻薯属6个种的DNA条形码进行研究,对4个DNA片段可用性,种内种间变异,barcode gap进行了分析,采用Treebased和BBA两种方法比较不同序列的鉴定能力。结果显示:单片段ITS正确鉴定率最高,片段组合rbcL+matK正确鉴定率最高。支持CBOL植物工作组推荐的条码组合rbcL+matK可作为蒟蒻薯属物种鉴定的标准条码,建议ITS片段作为候选条码。丝须蒟蒻薯Tacca integrifolia采自西藏的居群与马来西亚居群形成了2个不同的遗传分支,且两者在形态上也存在一定的差异,很可能是一个新种。

关键词: 蒟蒻薯属, DNA条形码, ITS, matK, rbcL, trnH-psbA

Abstract:

Tacca (Dioscoreaceae) is a small genus which is taxonomically difficult because of a lack of obvious morphologically differences among species. DNA barcoding is a new method of rapid species identification and discovery using short, standardized genes or DNA regions. In this study, four candidate DNA barcoding regions that three (rbcL, matK, trnHpsbA) from chloroplast genome and one (ITS) from the nuclear genome, were evaluated among 36 accessions representing 6 species of Tacca. The capability of those regions were evaluated by intra and interspecific divergence and barcode gap analyses, and the identification efficiency was assessed using Treebased and BBA methods. The results indicated that both ITS and the core barcode rbcL+matK proposed by CBOL exhibited the highest resolution as a single and combined data respectively. Based on overall performance: matK+rbcL can be considered as a potential barcode for identifying the species of Tacca, ITS can be used as a supplementary barcode. DNA barcoding revealed two distinct lineages of T.integrifolia distributed allopatrically in Tibet and Malaysia. And these two lineages with morphological variations may potentially represent new species.

Key words: Tacca, DNA barcoding, ITS, matK, rbcL, trnH-psbA

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