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Plant Diversity ›› 2012, Vol. 34 ›› Issue (01): 28-32.DOI: 10.3724/SP.J.1143.2012.11103

• 研究论文 • 上一篇    下一篇


 李婉莎, 王春涛, 胡向阳   

  1. 中国科学院昆明植物研究所,云南 昆明650201
  • 收稿日期:2011-07-05 出版日期:2012-02-25 发布日期:2011-10-15
  • 基金资助:

    中国科学院百人计划项目与国家自然科学基金项目 (30871704, 30971452, 31170256)

Isolation and Identification of an Arabidopsis thaliana Mutant with Serration Leaf Margin

 LI  Wan-Sha, WANG  Chun-Tao, HU  Xiang-Yang   

  1. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2011-07-05 Online:2012-02-25 Published:2011-10-15


叶的极性建立直接决定叶的平展性发育,极性改变导致叶形态异常,影响植物体的各种正常生理活动。利用反向遗传学方法,从拟南芥基因激活标签突变体库中分离到一个叶片边缘锯齿状表型的突变体(命名为pCB1294),该突变体同时表现出叶表皮腺毛形态发育异常。通过TailPCR方法成功定位突变基因为At5g41663,该基因编码miR319b基因。Real time PCR显示,pCB1294突变体植株中miR319b基因的表达量是野生型(col)植株的11倍多。所得结果为进一步研究miRNA调控叶极性的分子机制和进一步分析miR319b与叶形态发生的关系奠定了基础。

关键词: 拟南芥, 突变体, 叶锯齿, Tail-PCR, miRNA


Leaf polarity determines leaf flatness development directly, and abnormal polarity usually results in many abnormal leaves, which subsequently affects many physiological functions of plants. So the normal leaf development is important to plants. Here, an abnormal serration leaf margin mutant with abnormal leaf trichome development, named pCB1294, was isolated from an activation tagging Arabidopsis mutant pool through reverse genetics. By TailPCR, the mutant gene loci At5g41663 encoding miR319b was successfully identified. Real time PCR shows the relative expression level of miR319b gene in the pCB1294 mutant is eleven times of higher than that of the wild (col). Our study lay the foundation for further studying the genetic mechanism of leaf polarity and investigating the interaction between miR319b and leaf morphology.

Key words: Arabidopsis thaliana, Mutant, Leaf margin serration, Tail-PCR, miRNA