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Plant Diversity ›› 2012, Vol. 34 ›› Issue (2): 164-170.DOI: 10.3724/SP.J.1143.2012.11129

• 研究论文 • 上一篇    下一篇


 赵娟, 陈琰, 惠圣铭, 屠唯一, 陈晨, 高菊芳   

  1. 上海师范大学生命与环境科学学院,上海200234
  • 收稿日期:2011-09-01 出版日期:2012-04-25 发布日期:2011-12-31
  • 基金资助:

    国家自然科学基金项目 (30530100, 30970268) 资助;上海师范大学校基金项目 (SK200828)

Genetic and Mapping Analysis of Arabidopsis thaliana Male Sterile Mutant filament no elongation

 ZHAO  Juan, CHEN  Yan, HUI  Sheng-Ming, TU  Wei-Yi, CHEN  Chen, GAO  Ju-Fang   

  1. College of Life Environment Sciences, Shanghai Normal University, Shanghai 200234, China
  • Received:2011-09-01 Online:2012-04-25 Published:2011-12-31


在T-DNA插入突变体Salk_118481株系的群体中,筛选到一株雄性不育突变体,用T-DNA序列上的一对引物进行PCR鉴定表明其基因组中没有TDNA插入。通过背景纯化与遗传分析发现该雄性不育突变体是由单个隐性基因控制的,引起不育的主要原因是在花药发育的第13~14期,花丝不能伸长以完成授粉,故该突变体命名为fne (filament no elongation)。利用图位克隆的方法对FNE基因进行了定位,结果表明FNE基因位于第五条染色体上分子标记MBD2和MMG4之间的97kb区间内。目前该区间内尚未见到控制花丝伸长基因的报道,因此,FNE基因是一个控制花丝伸长的新基因。

关键词: 拟南芥, 雄性不育, 花丝伸长, 图位克隆


To identify new genes important for anther development, we screened for malesterile mutants among a population of Arabidopsis ecotype Columbia (Col) mutagenized by TDNA insertion (provided by ARBC). A male sterile mutant line with normal vegetative and flora development but no seed yield was isolated from Salk_118481 line. TDNA insertion site identification showed that there were no TDNA sequences in the genome of the mutants. Genetic analysis indicated that the mutant was controlled by a single recessive nuclear gene named filament no elongation because the filament of the mutant remains very short at the 13-14 stage of anther development. The fne gene was mapped to a region of 97kb between the molecular makers MBD2 and MMG4 on chromosome 5 using mapbased cloning technique. No genes involved filament elongation were reported in this region, so we believe that FNE gene could be a new gene controlling filament elongation in Arabidopsis.

Key words: Arabidopsis thaliana, Male sterile, Filament elongation, Map-based cloning