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Plant Diversity ›› 2009, Vol. 31 ›› Issue (03): 269-278.DOI: 10.3724 SP.J.1143.2009.08218

• 研究论文 • 上一篇    下一篇

卤代酶与生物卤化反应进展

耿召良1 , 2 , 3 , 王浩鑫1 , 赵沛基1 , 郝小江1 , 曾 英1   

  1. 1 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室, 云南昆明 650204; 2 中国科学院研究生院, 北京 100049; 3 贵州省烟草科学研究所, 贵州贵阳 550003
  • 收稿日期:2008-12-04 出版日期:2009-06-25 发布日期:2009-06-25

Research Development on Halogenases and Biological Halogenation

GENG Zhao-Liang1 , 2 , 3 , WANG Hao-Xin1 , ZHAO Pei-Ji1 , HAO Xiao-Jiang1 , ZENG Ying1   

  1. 1 State Key Laboratory of Phytochemistry and Plant Resources in West China , Kunming Institute of Botany , Chinese Academy of Sciences, Kunming 650204 , China; 2 Graduate University of Chinese Academy of Sciences,
    Beijing 100049 , China ; 3 Tobacco Research Institute of Guizhou , Guiyang 550003 , China
  • Received:2008-12-04 Online:2009-06-25 Published:2009-06-25

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摘要： 卤化物在生物圈内广泛存在, 许多天然卤化物广泛应用在药理学领域。根据催化机理, 催化形成C-X 键的卤代酶( halogenases ) 主要分为两大类型:卤代过氧化物酶(haloperoxidases) 和黄素依赖型卤代酶( flavin-dependent halogenases) , 另外还有非血红素Fe ( Ⅱ )􊄯α-酮戊二酸盐依赖型卤代酶( non-heme FeII􊄯α-ketoglutarate
( aKG)-dependent halogenases) 、甲基卤代转移酶(methyl halide transferases) 和氟化酶(fluorinases ) 等。本文综述了目前已知的卤代酶的发现、分子作用机制和生物催化潜力。近年来, 卤代酶在生物卤化过程中的重要生物学功能已经引起了广泛关注。利用组合生物合成、定向进化等现代生物技术合成有价值的天然卤代衍生物将有广阔的应用前景。

关键词: 卤代酶, 生物卤化, 卤化物

Abstract: Halogenated molecules are widely distributed in the biosphere with a variety of uses in pharmacology. Halogenating enzymes have been identified for the biosynthesis of halogenated compounds by catalyzing the formation of carbonhalogen bond. According to their different reaction mechanisms, halogenating enzymes are divided into two main groups, haloperoxidases and flavin-dependent halogenases. In addition, other halogenases including non-heme FeII􊄯α-ketoglutarate (αKG)-dependent halogenases, methyl halide transferases and fluorinase were recently discovered. This review concentrates on the occurrence , mechanism and biocatalytic potential of the halogenating enzymes that are currently known. The biological importance of halogenating enzymes in biohalogenation has aroused wide concern. Meanwhile, using modern biotechnology, for example , combinatorial biosynthesis and directed evolution , the prospects for generating halogenated derivatives of valuable natural products would therefore appear very bright.

Key words: Halogenase

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