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Plant Diversity ›› 2009, Vol. 31 ›› Issue (04): 344-352.DOI: 10.3724 SP.J.1143.2009.09090

• 研究论文 • 上一篇    下一篇

ABA 信号转运调节的基因表达与源库动力学分析

魏开发1, 贾文锁2   

  1. 1 漳州师范学院生物科学与技术系, 福建漳州 363000; 2 中国农业大学农学与生物技术学院, 北京 100094
  • 收稿日期:2009-05-06 出版日期:2009-08-25 发布日期:2009-08-25
  • 通讯作者: 魏开发

Analysis of Gene Expression and Source-sink Dynamics in Transportation Regulation of ABA Signal Accumulation

WEI Kai-Fa1, JIA Wen-Suo2   

  1. 1 Department of Biological Sciences and Biotechnology , Zhangzhou Normal University , Zhangzhou 363000 , China ;
    2 College of Agronomy and Biotechnology , China Agricultural University , Beijing 100094 , China
  • Received:2009-05-06 Online:2009-08-25 Published:2009-08-25
  • Contact: WEI Kai-Fa1

摘要: 通过对拟南芥NCED3、AAO3 及SDR1 蛋白亚细胞定位分析及根系和叶片ABA 池的动态库变化研究,结果表明气孔运动的有效ABA 信号来自于保卫细胞之外, SDR 与ABA 前体加工和运输有关。胁迫处理后根系合成酶基因转录水平显著高于叶片, 但叶片ABA 水平是根系的10 倍以上, 离体叶片和附体叶片ABA含量测定表明, 叶片ABA 池的形成主要决定于根源ABA 的输入。氟啶酮药剂阻断和遮荫实验说明根系ABA 池受叶源类胡萝素前体供应影响。叶片ABA 水平受根源ABA 和叶源类胡萝素前体库双向转运调节,维管束组织系统可能协同和整合了这一复杂调节机制。该结论为逆境ABA 信号转递机制研究和操纵内源ABA 含量增强植物抗逆性的应用提供相关资料。

关键词: ABA 合成酶基因, 基因表达, 源库动力学, SE-CC 复合体, 转运调节

Abstract: Subcellular localization of AtNCED3 , AtAAO3 and AtSDR1 proteins, and dynamic changes of ABA levels in root and leaf were investigated in the present study . Results showed that the guard cells were not the main site of ABA biosynthesis, and SDR might play an important role in ABA precursor processing and transportation . The transcription level of ABA synthase gene in roots was significantly higher than that in leaves under dehydration condition , while the ABA concentration in leaf tissues were 10 times higher than root . Detection of ABA concentration in both detached leaves and attached leaves suggested that ABA accumulation in leaves was mainly derived from root-sourced ABA, it was confirmed that ABA synthesis in roots was affected by the supply of precursor carotenoids with the fluridone treatment and shading experiments. These data revealed that ABA levels in leaves were regulated by root-sourced ABA transportation and leaf-sourced carotenoids supply. Our present results showed that vascular system might be involved in the coordination and integration of this complex regulatory mechanism for ABA signal accumulation. The conclusion provided relevant information for exploring molecular mechanism of stress signal transduction, manipulating endogenous ABA levels, and enhancing plants resistance
against environmental stresses .

Key words: ABA synthase gene