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Plant Diversity ›› 2009, Vol. 31 ›› Issue (1): 15-20.DOI: 10.3724 SP.J.1143.2009.08074

• 研究论文 • 上一篇    下一篇

温光敏核不育水稻N28S 无花粉败育的显微结构观察

彭海峰1 , 万邦惠2 , 张桂权2 , 陆燕鹏2 , 周国权1 , 陈雄辉2   

  1. 1 华南农业大学生命科学学院, 广东广州 510642; 2 华南农业大学农学院, 广东广州 510642
  • 收稿日期:2008-04-24 出版日期:2009-02-25 发布日期:2009-02-25

Microstructure Observations of Pollenless Abortion in Thermoand Photoperiod-sensitive Genic Male Sterile Line N28S in Rice ( Oryza sativa)

PENG Hai-Feng1 , WAN Bang-Hui2 , ZHANG Gui-Quan2 , LU Yan-Peng2   

  1. 1 College of Life Science , South China Agricultural University , Guangzhou 510642 , China; 2 College of Agriculture, South China Agricultural University , Guangzhou 510642 , China
  • Received:2008-04-24 Online:2009-02-25 Published:2009-02-25

摘要: 采用石蜡切片和荧光显微技术观察了温光敏核不育水稻N28S 无花粉败育过程中的显微结构变化, 结果显示: N28S 的小孢子母细胞形成后细胞质变得稀薄, 一部分不能进行减数分裂, 一部分减数分裂阻滞在细线期或胞质分裂异常, 最终所有细胞液泡化解体消失。在此过程中, 还观察到小孢子母细胞在细线期胼胝质壁不产生或提早消失, 以及小孢子发育后期花药壁绒毡层的异常解体。认为N28S 的无花粉败育是由小孢子母细胞的细胞质异常引起的, 胼胝质壁和绒毡层的异常是结果而不是原因。

关键词: 水稻, 温光敏核不育性, 无花粉败育, 显微结构

Abstract: Using paraffin section method and fluorescence microscopy , anther microstructure changes of pollenless abortion were observed in thermo- and photoperiod-sensitive genic male sterile line N28S in rice. The results showed that the microsporocytes of N28S had a reduced cytoplasm. Some microsporocytes didn′t undergo meiosis, the other microsporocytes arrested meiotic division at the leptotene stage of meiosis or underwent abnormal cytokinesis . All abnormal microsporocytes and meiotic products became vacuolate and eventually degenerated, leaving empty anther locules . Moreover, the absence or premature degradation of callose wall around the microsporocytes and the abnormal degradation of tapetum in the microspore
development stage were also been observed . It is suggested that the abnormal behaviour of callose and tapetum may be a consequence of a deviation in microsporocyte development, and the pollenless abortion of N28S is possibly attributed to microsporocytes with reduced cytoplasm .

Key words: Thermo- and photoperiod-sensitive genic male sterility