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Plant Diversity ›› 2009, Vol. 31 ›› Issue (05): 408-414.DOI: 10.3724 SP.J.1143.2009.09134

• 研究论文 • 上一篇    下一篇

中国小麦微核心种质资源Psy 基因的等位变异

蔡 华1 , 2 , 马传喜2, 司红起2 , 乔玉强2   

  1. 1 滁州学院化学与生命科学系, 安徽滁州 239000; 2 安徽农业大学农学院, 安徽合肥 230036
  • 收稿日期:2009-07-14 出版日期:2009-10-25 发布日期:2009-10-25

Allelic Variation of Psy Gene in Chinese Wheat Micro-Core Collections

CAI Hua1 , 2 , MA Chuan-Xi2, SI Hong-Qi2 , QIAO Yu-Qiang2   

  1. 1 Department of Chemistry and Life Science, Chuzhou University, Chuzhou 239000 , China ;
    2 College of Agronomy , Anhui Agricultural University , Hefei 230036 , China
  • Received:2009-07-14 Online:2009-10-25 Published:2009-10-25
  • Contact: MA Chuan-Xi

摘要: 根据已发表的麦族植物Psy 基因序列的保守区设计引物Psy02 , 克隆小麦Psy 基因( 片段)。结果表明, Psy02 引物的扩增产物出现2 种带型: 196 bp 和233 bp , 序列分析表明两条特异条带涵盖了小麦Psy 基因第2 外显子全部序列, 相差的37 bp 为Psy 基因第2 内含子中的一段插入序列, 可反映不同黄色素含量(YPC) , 属小麦Psy 基因的等位变异。验证试验表明, 248 份小麦微核心种质中有153 份材料(占样品数的65. 7%) 扩增出196 bp 条带, 群体内YPC 均值7. 314mg kg - 1 , 属高YPC 范畴; 另有95 份材料(占样品总数的38. 3%) 扩增出233 bp 条带, 群体内YPC 均值为5 . 207mg kg- 1 , 属低YPC范畴, 方差分析表明二者YPC 差异达1%极显著水平差异, 说明上述37 bp 的插入序列是导致小麦品种间YPC 产生差异的原因之一, 因此该引物扩增的Psy 基因对小麦YPC 具有显著影响, 引物Psy02 是对小麦YPC 进行分子鉴定的重要标记。

关键词: 小麦, 微核心种质, Psy 基因, 等位变异

Abstract: Primer Psy02 was designed according to conservative areas of Psy gene sequence of Ttiticum, and partial sequence of Psy gene of wheat was cloned . The results showed that , two different PCR products were amplified with 196 bp and 233 bp, both of which covered whole sequence of the second exon of wheat Psy gene, and the 37 bp difference between two bands was an insertion sequence of the second exon of Psy gene, which display different YPC in wheat . Verification test showed , that among 248 wheat micro core collections, 153 samples amplified 196 bp band while the rest samples
amplified 233 bp band which accounted for 61.7% and 38.3% respectively. The YPC average value of 153 samples was 7.314mg kg-1 which belong to high YPC rang and that of 95 smaples was 5.207mg kg-1 which belong to low YPC rang. Variance analysis showed that the YPC difference reached 1% significant level , which proved the 37 bp insertion sequence was one of the reasons of different YPC in cultivars , in a word , the YPC of wheat was significantly affected by the Psy gene and, primer Psy02 was an important molecular marker to identifying YPC .

Key words: Wheat