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Plant Diversity ›› 2008, Vol. 30 ›› Issue (03): 340-344.DOI: 10.3724 SP.J.1143.2008.07203

• 研究论文 • 上一篇    下一篇

一个巴西橡胶树钙调素基因假基因的识别*

张治礼1 , 2 , 苏火生1 , 李维国3   

  1. 1 中国热带农业科学院热带生物技术研究所, 海南海口 571101; 2 海南省农业科学院, 海南海口 571100; 3 中国热带农业科学院橡胶研究所, 海南儋州 571737
  • 收稿日期:2007-09-14 出版日期:2008-06-25 发布日期:2008-06-25
  • 通讯作者: 张治礼

Identification of a Pseudogene of Calmodulin Gene in Hevea brasiliensis (Euphorbiaceae)

ZHANG Zhi-Li1 , 2 , SU Huo-Sheng1 , LI Wei-Guo3   

  1. 1 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101 , China; 2 Hainan Academy of Agricultural Sciences, Haikou 571101 , China ; 3 Rubber Research , Institute Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737 , China

  • Received:2007-09-14 Online:2008-06-25 Published:2008-06-25
  • Contact: ZHANG Zhi-Li

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摘要： 以巴西橡胶树( Hevea brasiliensis) 基因组DNA 为模板, 根据胶乳钙调素基因HbCaM 序列设计引物,利用PCR 方法克隆获得了1 319 bp 和447 bp 的两个DNA 片段。序列分析表明, 1 319 bp 的DNA 长片段为胶乳钙调素基因HbCaM 的DNA 片段, 含有两个外显子( 77 bp 和373 bp) 和1 个内含子( 869 bp) , 包含了HbCaM cDNA 编码区447 bp 的全部序列; 447 bp 的DNA 小片段与HbCaM cDNA 核苷酸序列同源性高达98%, ORF 分析发现, 位于406 bp 处的碱基C 突变为T, 即三联体密码CAG 突变为终止子TAG 使翻译提前终止, 其余5 个差异碱基都不影响或改变正常的翻译。推测此447 bp 的DNA 片段为巴西橡胶树钙调素Hb-CaM 基因的假基因, 命名为HbCaMP1。

关键词: 巴西橡胶树, 钙调素基因, 假基因

Abstract: Employing a pair of primers designed from cDNA of a HbCaM gene, two 1371 bp and 447 bp-length fragments were isolated from genome DNA in Hevea brasiliensis. Sequence analysis showed that the longer fragment was the HbCaM gene with 2 exons ( including the 77 bp and 373 bp-length fragments) and an 869 bp-length intron, and the shorter fragment had 98% identity with cDNA sequence of HbCaM gene. Compared to cDNA of the HbCaM gene, 6 mutated nucleotides were identified in the 447 bp-length fragment, in which T mutated from C at 406 bp site led to the occurrence of stop codon TAG in advance . The 447 bp- length fragment was hereby suggested to be a pseudogene of the HbCaM gene in H. brasiliensis, named HbCaMP1, which was the first pseudogene in identified H. brasiliensis geonome and the first homological pseudogene of CaM genes isolated in plant .

Key words: Hevea brasiliensis

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