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Plant Diversity ›› 2008, Vol. 30 ›› Issue (04): 457-463.DOI: 10.3724 SP.J.1143.2008.07228

• 研究论文 • 上一篇    下一篇



  1. 福建师范大学生命科学学院发育与神经生物学重点实验室, 福建福州 350108
  • 收稿日期:2007-10-08 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 王逸群

Transformation of Human Alpha-lactalbumin Gene into Tobacco and Improvement of Cysteine Contents in Transgenic Tobacco Plants

WANG Yi-Qun   

  1. Laboratory of Developmental and Neural Biology , College of Life Sciences, Fujian Normal University , Fuzhou 350108 , China
  • Received:2007-10-08 Online:2008-08-25 Published:2008-08-25
  • Contact: WANG Yi-Qun

摘要: 将人α-乳清白蛋白基因构建到植物表达载体上, 通过农杆菌介导采用叶盘法将人α-乳清白蛋白基因导入到烟草中, 经过PCR 和Southern blot 分析表明: 人α-乳清白蛋白基因已经整合到烟草基因组中; 经过RT-PCR 和GUS 组织化学染色证明: 人α-乳清白蛋白基因得到了表达。测定了9 株转基因烟草叶片半胱氨酸含量, 大部分植株有着明显的提高, 最高幅度达到了318.02% , 半胱氨酸含量平均提高了166.40%。

关键词: 人α-乳清白蛋白基因, 转基因烟草, 半胱氨酸, 提高

Abstract: The plant expression vector containing human alpha-lactalbumin gene was constructed and its gene was transformed into tobacco using leaf disc mediated by Agrobacterium tumefaciens LBA 4404 as a vehicle. PCR and Southern blot analyses showed that human alpha-lactalbumin gene was integrated into the tobacco genome. RT-PCR and histochemical staining for GUS activity demonstrated that human alpha-lactalbumin gene was expressed in transgenic tobacco plants. Further analysis of the data indicated that the average of cysteine contents in 9 transgenic tobacco plants was greatly increased by 166.40% besides one of them which was increased by 318.02%

Key words: Human alpha-lactalbumin gene