ESI-MS MS方法检测拟南芥膜脂分子对机械伤害的响应

Plant Diversity ›› 2007, Vol. 29 ›› Issue (06): 671-676.

ESI-MS MS方法检测拟南芥膜脂分子对机械伤害的响应

凌立贞^{1 , 2 , 3} , 李唯奇^{3 , 4} , 徐正君^{1 , 2}

1 四川农业大学水稻研究所, 四川温江 611130; 2 四川农业大学西南作物基因资源与遗传改良教育部重点实验室, 四川雅安 625014 ; 3 中国科学院昆明植物研究所, 云南昆明 650204 ;4 红河学院生物系, 云南蒙自 661100

收稿日期:2007-03-05 出版日期:2007-12-25 发布日期:2007-12-25
通讯作者: 李唯奇,徐正君

Profiling the Changes of Lipid Molecular Species in Response to
Wounding in Arabidopsis thaliana (Cruciferae) by ESI-MS MS

LING Li-Zhen^{1 , 2 , 3} , LI Wei-Qi^{3 , 4}, XU Zheng-Jun^{1 , 2}

1 Rice Research Institute, Sichuan Agricultural University , Wenjiang 611130 , China; 2 Key Laboratory of Southwest Crop Gene
Resources and Improvement , Ministry of Education , Sichuan agricultural University, Yaan 625014 , China ;
3 Kunming Institute of Botany , Chinese Academy of Sciences, Kunming 650204 , China ;
4 Department of Biology , Honghe University, Mengzi 661100 , China

Received:2007-03-05 Online:2007-12-25 Published:2007-12-25
Contact: LI Wei-Qi,XU Zheng-Jun

摘要/Abstract

摘要： 利用一种灵敏的、基于ESI-MS􊄯MS ( electrospray ionization tandem mass spectrometry) 的脂类组学方法,测定了机械伤害诱导的拟南芥6 种磷 (phospholipids) 、2 种糖脂(glycolipids) 、3 种溶血磷脂( lysophospholipids)和约120 种脂类分子的变化, 探索了膜脂响应机械伤害的基本趋势。结果表明, 机械伤害后磷脂酸( phosphatidic acid , PA) 和3 种溶血磷脂显著升高, 而叶绿体膜上的糖脂减少; 在测量的1 小时范围内, 不同脂类水解产生的磷脂酸分子的增加速度和强度不同, 反映出它们经历了不同的生化过程。具体表现为:
(1 ) 叶绿体膜脂磷脂酰甘油(phosphatidylglycero , PG) 分子34∶4 PG 水解的产物磷脂酸分子34∶4 PA 的积累速度明显慢于其它磷脂酸分子; (2) 磷脂酸分子34∶6 PA 仅有少量的积累, 其可能是由叶绿体膜脂单半乳糖二酰甘油(monogalactosyldiacylglycerol, MGDG) 。分子34∶6 MGDG 和双半乳糖二酰甘油( digalactosyldiacylglycerol, DGDG) 分子34∶6 DGDG 水解产生, 然而这两种糖脂含量明显下降, 说明它们有可能还参与了其它的反应。脂类的摩尔百分组成没有剧烈的变化。

关键词: 机械伤害, 脂类分子, 脂类组学

Abstract: This paper employed a sensitive approach based on electrospray ionization tandem mass spectrometry (ESI-MS􊄯MS) to comprehensively analyze lipid composition changes in response tomechanical wounding in Arabidopsis thaliana.The changes of 6 phospholipid classes, 2 glycolipid classes, and 3 lysophospholipid, and 120 lipid molecular species in total depicted the basic
tendency of membrane lipids in response to wounding for 0 , 15, 30 , and 60min in Arabidopsis thaliana . The results showed that wounding can lead to increase phosphatidic acid (PA) and 3 lysophospholipid and decrease glycolipids of the major chloroplast plastidic lipids . The increasing speed and intensity of PA produced by various lipids are very different , which shows those lipids have experienced different biochemistry processes . (1) The accumulation of 34∶4 PA derived from plastidic lipid 34∶4 PG (phosphatidylglycerol) is significantly slower than that of other PA species; (2) 34∶6 PA accumulated a little and possibly derived from the 34∶6 MGDG or DGDG . However , the content of both glycolipids has apparently decreased, which indicated they are possibly involved in other reactions. No remarkable changes of lipid mole percentage were observed .

Key words: Mechanical wounding

中图分类号:

Q 945

凌立贞1 , 2 , 3 , 李唯奇3 , 4 , 徐正君1 , 2 . ESI-MS MS方法检测拟南芥膜脂分子对机械伤害的响应[J]. Plant Diversity, 2007, 29(06): 671-676.

LING Li-Zhen^{, ,} , LI Wei-Qi^,, XU Zheng-Jun^,. Profiling the Changes of Lipid Molecular Species in Response to
Wounding in Arabidopsis thaliana (Cruciferae) by ESI-MS MS[J]. Plant Diversity, 2007, 29(06): 671-676.

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