菘蓝花药培养及单倍体的诱导

doi:10.3724/SP.J.1143.2011.10155

Plant Diversity ›› 2011, Vol. 33 ›› Issue (2): 225-228.DOI: 10.3724/SP.J.1143.2011.10155

菘蓝花药培养及单倍体的诱导

陕西师范大学药用资源与天然药物化学教育部重点实验室西北濒危药材资源开发国家工程实验室, 陕西西安 710062

收稿日期:2010-09-14 出版日期:2011-04-25 发布日期:2010-12-20
基金资助:
陕西省科技攻关计划（2009K19-07）

Anther Culture and Haploid Induction from Isatis indigotica (Cruciferae)

Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry,
National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in
Northwest of China, Shaanxi Normal University, Xi′an 710062, China

Received:2010-09-14 Online:2011-04-25 Published:2010-12-20

摘要/Abstract

摘要：

探讨菘蓝花药处于单核晚期的形态指标，并以适宜发育时期的花药为外植体，进行花药培养及单倍体诱导。实验结果表明，4℃低温处理2d后，在含有6-BA 0.5mg·L-1和NAA 1.0mg·L-1的MS培养基上，花药愈伤组织的诱导率为23.35%；将其转接到MS附加 6-BA 1.0mg·L-1，NAA 0.5mg·L-1的分化培养基上，80.00%以上的愈伤组织可以诱导产生不定芽；再将分化出的试管苗转接到1/2MS+NAA 1.0mg·L-1的生根培养基上，3d左右即可获得完整植株。经叶边缘压片检查染色体数目，花药培养所得的弱小绿苗为单倍体植株。

关键词: 菘蓝, 花药培养, 单倍体

Abstract:

Anther culture and haploid induction were carried out from Isatis indigotica. The experimental results showed that cold pretreatment could increase the induction rate of callus. The induction rate of callus was 23.35% after 2 days of cold pretreatment. Then the calluses were successively transferred to differentiation medium (MS+BA 0.5mg·L-1+ NAA 1.0mg·L-1) and rooting medium (1/2MS+NAA 1.0mg·L-1). The complete plants were obtained 3 days later. Among which, those weak and green plantlets were haploids with 7 chromosomes.

Key words: Isatis indigotica, Anther culture, Haploid

中图分类号:

Q 943.1

李焘, 林文媛, 王喆之. 菘蓝花药培养及单倍体的诱导[J]. Plant Diversity, 2011, 33(2): 225-228.

LI Tao, LIN Wen-Yuan, WANG Zhe-Zhi. Anther Culture and Haploid Induction from Isatis indigotica (Cruciferae)[J]. Plant Diversity, 2011, 33(2): 225-228.

参考文献

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菘蓝花药培养及单倍体的诱导

Anther Culture and Haploid Induction from Isatis indigotica (Cruciferae)

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