增强UV-B辐射对小麦根尖分裂期细胞肌动蛋白的影响

doi:10.3724/SP.J.1143.2011.10180

Plant Diversity ›› 2011, Vol. 33 ›› Issue (3): 306-310.DOI: 10.3724/SP.J.1143.2011.10180

增强UV-B辐射对小麦根尖分裂期细胞肌动蛋白的影响

陈慧泽, 翟菁如, 杜美婷, 韩榕

山西师范大学生命科学学院，山西临汾041004

收稿日期:2010-10-21 出版日期:2011-06-25 发布日期:2011-03-09
基金资助:
国家自然科学基金资助项目(30671061)增强UV-B辐射与He-Ne激光对小麦细胞分束分裂影响机制的研究；山西省自然科学基金资助项目(200811059)作物相关基因的定位、克隆及转化

Influence of Enhanced UV-B Radiation on F-actin in Wheat Division Cells

CHEN Hui-Ze, ZHAI Jing-Ru, DU Mei-Ting, HAN Rong

College of Life Science, Shanxi Normal University, Linfen 041004, China

Received:2010-10-21 Online:2011-06-25 Published:2011-03-09

摘要/Abstract

摘要：

以增强UV-B (10.08kJ·m-2·d-1)辐射后的小麦根尖细胞为材料，异硫氰酸荧光素标记的鬼笔环肽(FITC-Ph)为探针，利用激光共聚焦扫描显微镜，观察分析小麦根尖分裂期细胞肌动蛋白在分裂周期的分布及形态变化。结果表明：UV-B辐射处理后，间期细胞肌动蛋白纤丝排列紊乱；前期细胞周质中肌动蛋白纤丝变为短的片段，点状荧光随机分布在周质；中期细胞荧光强度明显减弱，明亮的肌动蛋白片段消失，点状荧光消失；有丝分裂后期到末期，成膜体区域肌动蛋白纤丝或成弥散状或完全消失，且出现落后染色体、染色体桥、不均等分裂及三束分裂等染色体畸变类型和异常分裂现象。

关键词: 增强UV-B, 小麦, 分束分裂细胞, 肌动蛋白, 激光共聚焦扫描显微镜

Abstract:

The distribution of F-actin was examined by confocal laser scanning microscopy (CLSM) in the wheat root-tip cells under the enhanced ultraviolet-B (10.08kJ·m-2·d-1) radiation treated with FITC-Phalloidin. The results showed that under the condition of the enhanced ultraviolet-B radiation, distribution of actin MFs were disordered in interphase cells; The arrays of the F-actin disintegrated into short fragments of random during prophase; Fluorescence intensity significantly decreased and bright fragments of microfilaments disappeared in Metaphase cells; Actin MFs disappeared in the phragmoplast regions when cells were in anaphase and telophase time. And some types of chromosome aberration were induced, including Chromosomal logging, chromosomal bridge and partition-bundle division.

Key words: Enhanced UV-B radiation, Wheat, Partition bundle division cells, Actin, Confocal laser scanning microscope (CLSM)

中图分类号:

Q 942

陈慧泽, 翟菁如, 杜美婷, 韩榕. 增强UV-B辐射对小麦根尖分裂期细胞肌动蛋白的影响[J]. Plant Diversity, 2011, 33(3): 306-310.

CHEN Hui-Ze, ZHAI Jing-Ru, DU Mei-Ting, HAN Rong. Influence of Enhanced UV-B Radiation on F-actin in Wheat Division Cells[J]. Plant Diversity, 2011, 33(3): 306-310.

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增强UV-B辐射对小麦根尖分裂期细胞肌动蛋白的影响

Influence of Enhanced UV-B Radiation on F-actin in Wheat Division Cells

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