云南悬钩子蔷薇NBSLRR类抗病基因同源克隆与分析

doi:10.3724/SP.J.1143.2012.11057

Plant Diversity ›› 2012, Vol. 34 ›› Issue (01): 56-62.DOI: 10.3724/SP.J.1143.2012.11057

云南悬钩子蔷薇NBSLRR类抗病基因同源克隆与分析

陈玲1、2, 张颢1, 邱显钦1, 晏慧君1, 王其刚1, 蹇洪英1, 唐开学1

1 云南省农业科学院花卉研究所花卉育种重点实验室，云南昆明650205；
2 云南大学生命科学学院，云南昆明650091

收稿日期:2011-04-02 出版日期:2012-02-25 发布日期:2011-09-23
基金资助:
国家自然科学基金项目 (31160403)；云南省应用基础研究计划项目 (2011FB124)；科技部863项目(2011AA100208)；云南省攻关项目 (2011BB013)；农业部948项目 (2011G17)

Cloning and Analysis of NBSLRR Type Disease Resistance Gene Analogs from Rosa rubus in Yunnan

CHEN Ling-1、2, ZHANG Hao-1, QIU Xian-Qin-1, YAN Hui-Jun-1, WANG Ji-Gang-1, JIAN Hong-Ying-1, TANG Kai-Xue-1

1 Flower Research InstitutFlower Breeding Key Labe, Yunnan of Agricultural Sciences, Kunming 650205, China;
2 College of Life Sciences, Yunnan University, Kunming 650091, China

Received:2011-04-02 Online:2012-02-25 Published:2011-09-23

摘要/Abstract

摘要：

为研究云南野生蔷薇属中的NBS类抗病基因，根据已知抗病基因NBSLRR序列中的保守区域设计简并引物，利用RTPCR技术从云南悬钩子蔷薇中进行体外扩增，获得了对应区域的cDNA片段，回收、克隆这些特异片段，测序分析，共得到4个含有NBSLRR保守结构域的抗病基因同源序列(RGAs)，分别命名为AC9、AC39、AC50和AC68。它们与已报道的11个NBS类抗病基因相应区段的氨基酸序列相似性为5.4%~79.2%，其中这4个RGAs片段与Mi、RPS2、Pib和RPM1基因聚为一类。表明这4条RGAs序列可进一步用作悬钩子蔷薇抗病候选基因的分子筛选及遗传图谱的构建。

关键词: 悬钩子蔷薇, 保守区域, 抗病基因同源序列, 简并引物

Abstract:

In the study of NBS resistance genes of Yunnan wild Rosa, the degenerate oligonucleotide primers were designed according to the conserved motifs NBSLRR of most plant resistance (R) genes. The cDNA fragments were isolated from Rosa rubus by reverse transcription PCR (RTPCR). Then four resistance gene analogs (RGAs) were sequenced and named as AC9, AC39, AC50 and AC68, which contain NBSLRR domain. The sequence of each RGAs was compared with NBS region of eleven resistance genes reported before, and the similarities of aminoacid sequences ranged from 5.4% to 79.2%, and these four RGAs were clustered a group with Mi、RPS2、Pib and RPM1 gene. These results showed that the four RGAs will be used as molecular markers for screening candidate disease resistance genes and genetic map construction of Rosa rubus in the future.

Key words: Rosa rubus, Conserved motif, Resistance gene analogs (RGAs), Degenerate oligonucleotide primers

中图分类号:

Q 78

陈玲1、2, 张颢1, 邱显钦1, 晏慧君1, 王其刚1, 蹇洪英1, 唐开学1. 云南悬钩子蔷薇NBSLRR类抗病基因同源克隆与分析[J]. Plant Diversity, 2012, 34(01): 56-62.

CHEN Ling-, ZHANG Hao-, QIU Xian-Qin-, YAN Hui-Jun-, WANG Ji-Gang-, JIAN Hong-Ying-, TANG Kai-Xue. Cloning and Analysis of NBSLRR Type Disease Resistance Gene Analogs from Rosa rubus in Yunnan[J]. Plant Diversity, 2012, 34(01): 56-62.

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云南悬钩子蔷薇NBSLRR类抗病基因同源克隆与分析

Cloning and Analysis of NBSLRR Type Disease Resistance Gene Analogs from Rosa rubus in Yunnan

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