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Plant Diversity ›› 2012, Vol. 34 ›› Issue (5): 519-524.DOI: 10.3724/SP.J.1143.2012.12028

• 研究论文 • 上一篇    

杨柳田头菇B交配型基因信息素受体片段的克隆与分析

 陈卫民1、2、3, 柴红梅1、2、3, 张小雷1、2、3, 李树红1、2、3, 赵永昌1、2、3   

  1. 1 云南省农业科学院生物技术与种质资源研究所,云南 昆明650221;2 云南省农业生物技术重点实验室,
    云南 昆明650221;3 农业部西南作物基因资源与种质创制重点实验室,云南 昆明650221
  • 收稿日期:2012-03-07 出版日期:2012-10-25 发布日期:2012-07-11
  • 基金资助:

    国家自然科学基金项目 (31101591), 云南省应用基础研究计划 (2011FZ214) 及现代农业产业技术体系建设专项资金“国家食用菌产业技术体系” (CARS24)

Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola (Strophariaceae)

 CHEN  Wei-Min-1、2、3, CHAI  Hong-Mei-1、2、3, ZHANG  Xiao-Lei-1、2、3, LI  Shu-Hong-1、2、3, ZHAO  Yong-Chang-1、2、3   

  1. 1 Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650221, China;
     2 Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming 650221, China; 3 Key Lab of Southwestern
    Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650221, China
  • Received:2012-03-07 Online:2012-10-25 Published:2012-07-11

摘要:

利用简并PCR及DNA步移法,从杨柳田头菇Agrocybe salicacola YAASM0711菌株中扩增得到了一个4231bp的核酸片段。经过比对及序列预测,所获得序列中含有杨柳田头菇交配型编码基因中的信息素受体部分,其序列长度为1194bp,包含4个内含子,5个外显子的长度分别为217bp,113bp,67bp,138bp,449bp。拼接后的ORF全长984bp,编码327个氨基酸残基。该序列与灰盖鬼伞Coprinus cinerea、双色蜡蘑Laccaria bicolor信息素受体氨基酸序列较为相似,含有7个跨膜区。信息素受体遗传进化分析显示,其与多个物种信息素受体聚集在一起,可能与真菌信息素受体的多种起源有关。

关键词: 杨柳田头菇, 信息素受体, 交配型, 简并PCR, 氨基酸序列

Abstract:

A 4231bp DNA fragment of B mating type pheromone receptor from strain YAASM0711 of Agrocybe salicacola was obtained by using degenerate PCR and DNA walking techniques. The result of alignment and structure prediction of DNA sequences showed that one 1194bp nucleotides gene ASRcb1 encoding B mating pheromone receptor was found, including four introns(72bp, 49bp, 48bp and 41bp) and five extrons (217bp, 113bp, 67bp, 138bp and 449bp). The spliced open reading frame (ORF) contains 984bp nucleotides encoding 327 amino acid residues, and includes seven transmembrane protein regions as in its similar sequences of Coprinus cinerea and Laccaria bicolor pheromone receptors. The genetic evolution of pheromone receptor showed ASRcb1 was clustered with more receptors, which suggested multiple ways of evolution occurred in fungi.

Key words: Agrocybe salicacola, Pheromone receptor, Mating type, Degenerate PCR, Amino acid sequence

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