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Plant Diversity ›› 2012, Vol. 34 ›› Issue (6): 607-613.DOI: 10.3724/SP.J.1143.2012.12162

• 研究论文 • 上一篇    下一篇


 袁庆军1, 张文婧1、2, 姜丹1、2, 张永清2, 马超一1, 黄璐琦1   

  1. 1 中国中医科学院中药研究所,北京100700; 2 山东中医药大学药学院,山东 济南250355
  • 收稿日期:2012-11-20 出版日期:2012-12-25 发布日期:2012-11-28
  • 基金资助:

    国家科技部科技基础工作专项项目;国家高科技研究发展计划 (863计划) (2012AA021801);中国科学院大科学装置开放研究项目 (2009-LSFGBOWS-01);国家自然科学基金重点项目 (81130070);中国中医科学院中药研究所基本科研业务费自主选题项目 (2011ADXK-01)

On the Methods and Principles of Molecular Identification of Chinese Herbs

YUAN  Qing-Jun-1, ZHANG  Wen-Jing-1、2, JIANG  Dan-1、2, ZHANG  Yong-Qing-2, MA  Chao-Yi-1, HUANG  Lu-Qi-1   

  1. 1 Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China;
    2 College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • Received:2012-11-20 Online:2012-12-25 Published:2012-11-28



关键词: 中药, 分子鉴定, 真伪品, 正品和替代品, 多基源, 产地鉴别


Accurate identification of herbal medicinal materials is relevant to the safety of human life and economic interests. Traditional identification methods, including sensory evaluation, microscopic identification, physical and chemical identification, all have their limitations. Molecular identification brings a new opportunity for accurate identification of herbal medicinal materials. However, prior to its wide adoption, the methods and principles of molecular identification should be fully discussed. In this paper, we proposed a set of new methods for molecular authentication of herbal medicinal materials: 1) Identification between authenticity and adulteration by using specific polymerase chain reaction; 2) Identification between official herb and substitute by using the method of DNA barcoding; 3) Identification among multiple species of one official herbs by constructing genealogy among closelyrelated species based on population genetics; 4) Identification among herbs of different geographical origins by phylogeographybased analysis. For those that can not be identified by above four methods, more rapidly evolved markers such as microsatellite should be employed and individualbased analysis could be adopted.

Key words: Herbal medicines, Molecular identification, Authenticity and adulteration, Official herb and substitute, Multiple species origin, Different regional origins