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Plant Diversity ›› 2013, Vol. 35 ›› Issue (2): 170-172.DOI: 10.7677/ynzwyj201312093

• 研究论文 • 上一篇    下一篇

芒属植物荻愈伤组织的诱导与植株再生

 王青云, 程红焱, 王伟青, 宋松泉   

  1. 中国科学院植物研究所，北京100093
  • 收稿日期:2012-06-21 出版日期:2013-03-25 发布日期:2012-08-21
  • 作者简介:国家科技支撑计划 (2012BAC01B05)

Efficient Induction of Callus and Plant Regeneration from Miscanthus sacchariflorus Young Ear

 WANG  Qing-Yun, CHENG  Hong-Yan, WANG  Wei-Qing, SONG  Song-Quan   

  1. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2012-06-21 Online:2013-03-25 Published:2012-08-21
  • Supported by:

    国家科技支撑计划 (2012BAC01B05)

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摘要：

利用资源植物荻（Miscanthus sacchariflorus）的幼穗作为外植体，通过愈伤组织途径建立了植株再生体系。结果表明：在附加600mg·L-1脯氨酸、500mg·L-1水解酪蛋白、2.0mg·L-1 2, 4D和0.1mg·L-1 6BA的MS培养基上能高效诱导出生长良好的愈伤组织；将其转移到MS+1.5mg·L-1 6BA培养基上诱导产生芽，再转移到1/2 MS+0.25mg·L-1 NAA+0.25mg·L-1 IAA培养基上生根后，可发育为生长健壮的植株。

关键词: 愈伤组织途径, 芒属植物荻, 再生体系, 幼穗

Abstract:

 Plants belonging to the genus Miscanthus are considered promising bioenergy crops. Young ears of Miscanthus sacchariflorus (Maxim.) Benth. were used as explants, and its regeneration system was successfully established via callus pathway. The results showed that Murashige and Skoog (MS) medium containing 600mg·L-1 proline, 500mg·L-1 hydrolyzed casein, 2.0mg·L-1 2, 4D and 0.1mg·L-1 6BA was an optimal medium for induction and growth of callus from young ears. The shoots were induced on MS medium containing 1.5mg·L-1 6BA via callus. The roots were induced after shoots were transferred to 1/2 MS medium containing 0.25mg·L-1 NAA and 0.25mg·L-1 IAA, after that, the young plants were cultivated and grew well on the nutrient soil.

Key words:  Callus pathway, Miscanthus sacchariflorus, Regeneration system, Young ear

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