Functional characterization of the Arabidopsis SERRATE under salt stress

doi:10.1016/j.pld.2020.06.010

Plant Diversity ›› 2021, Vol. 43 ›› Issue (01): 71-77.DOI: 10.1016/j.pld.2020.06.010

Functional characterization of the Arabidopsis SERRATE under salt stress

Minghui Mou^a,c, Qijuan Wang^a,c, Yanli Chen^a,c, Diqiu Yu^a, Ligang Chen^a,b

a CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China;
b Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China;
c College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

收稿日期:2020-02-12 修回日期:2020-06-11 出版日期:2021-02-25 发布日期:2021-03-25
通讯作者: Diqiu Yu, Ligang Chen
基金资助:
This work was supported by the National key R & D plan (2016YFD0101006), Natural Science Foundation of China (31671275), Candidates of the Young and Middle-Aged Academic Leaders of Yunnan Province (2015HB094), Yunnan Fundamental Research Projects (grant NO. 2017FB047 and 2019FA010).

Functional characterization of the Arabidopsis SERRATE under salt stress

Minghui Mou^a,c, Qijuan Wang^a,c, Yanli Chen^a,c, Diqiu Yu^a, Ligang Chen^a,b

a CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China;
b Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China;
c College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

Received:2020-02-12 Revised:2020-06-11 Online:2021-02-25 Published:2021-03-25
Contact: Diqiu Yu, Ligang Chen
Supported by:
This work was supported by the National key R & D plan (2016YFD0101006), Natural Science Foundation of China (31671275), Candidates of the Young and Middle-Aged Academic Leaders of Yunnan Province (2015HB094), Yunnan Fundamental Research Projects (grant NO. 2017FB047 and 2019FA010).

摘要/Abstract

摘要： SERRATE (SE) plays critical roles in RNA metabolism and plant growth regulation. However, its function in stresseresponse processes remains largely unknown. Here, we examined the regulatory role of SE using the se-1 mutant and its complementation line under saline conditions. The expression of SE was repressed by salt treatment at both mRNA and protein levels. After treatment with different NaCl concentrations, the se-1 mutants showed increased sensitivity to salinity. This heightened sensitivity was evidenced by decreased germination, reduced root growth, more serious chlorosis, and increased conductivity of the mutants compared with the wild type. Further analysis revealed that SE regulates the pre-mRNA splicing of several well-characterized marker genes associated with salt stress tolerance. Our data thus imply that SE may function as a key component in plant response to salt stress by modulating the splicing of salt stress-associated genes.

关键词: SERRATE, Salt stress, Pre-mRNA alternative splicing

Abstract: SERRATE (SE) plays critical roles in RNA metabolism and plant growth regulation. However, its function in stresseresponse processes remains largely unknown. Here, we examined the regulatory role of SE using the se-1 mutant and its complementation line under saline conditions. The expression of SE was repressed by salt treatment at both mRNA and protein levels. After treatment with different NaCl concentrations, the se-1 mutants showed increased sensitivity to salinity. This heightened sensitivity was evidenced by decreased germination, reduced root growth, more serious chlorosis, and increased conductivity of the mutants compared with the wild type. Further analysis revealed that SE regulates the pre-mRNA splicing of several well-characterized marker genes associated with salt stress tolerance. Our data thus imply that SE may function as a key component in plant response to salt stress by modulating the splicing of salt stress-associated genes.

Key words: SERRATE, Salt stress, Pre-mRNA alternative splicing

Minghui Mou, Qijuan Wang, Yanli Chen, Diqiu Yu, Ligang Chen. Functional characterization of the Arabidopsis SERRATE under salt stress[J]. Plant Diversity, 2021, 43(01): 71-77.

图/表 6

参考文献 38

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尺度 Scale	MPD平均随机值 Mean MPD randomization	MPD平均实际测量值 Mean MPD analysis	标准差 SD	净相关指数 NRI	零模型 Null model
10 m×10 m	178.13	262.55	43.57	-1.97	约束型 Constrained
10 m×10 m	263.58	262.55	84.12	0.03	非约束型 Unconstrained
20 m×20 m	196.36	288.74	41.59	-2.20	约束型 Constrained
20 m×20 m	286.36	288.74	71.55	-0.01	非约束型 Unconstrained
25 m×25 m	201.98	297.13	40.38	-2.27	约束型 Constrained
25 m×25 m	293.39	297.13	68.11	-0.04	非约束型 Unconstrained

尺度 Scale	MPD平均随机值 Mean MPD randomization	MPD平均实际测量值 Mean MPD analysis	标准差 SD	净相关指数 NRI	零模型 Null model
10 m×10 m	178.13	262.55	43.57	-1.97	约束型 Constrained
10 m×10 m	263.58	262.55	84.12	0.03	非约束型 Unconstrained
20 m×20 m	196.36	288.74	41.59	-2.20	约束型 Constrained
20 m×20 m	286.36	288.74	71.55	-0.01	非约束型 Unconstrained
25 m×25 m	201.98	297.13	40.38	-2.27	约束型 Constrained
25 m×25 m	293.39	297.13	68.11	-0.04	非约束型 Unconstrained

尺度 Scale	MNND平均随机值 Mean MNND randomization	MNND平均实际测量值 Mean MNND analysis	标准差 SD	最近邻体指数 NTI	零模型 Null model
10 m×10 m	172.63	238.72	55.80	-1.110	约束型 Constrained
10 m×10 m	231.88	238.72	89.65	0.007	非约束型 Unconstrained
20 m×20 m	141.09	199.81	49.40	-1.170	约束型 Constrained
20 m×20 m	188.50	199.81	74.82	-0.024	非约束型 Unconstrained
25 m×25 m	133.57	189.81	47.05	-1.180	约束型 Constrained
25 m×25 m	179.78	189.81	69.60	-0.034	非约束型 Unconstrained

尺度 Scale	MNND平均随机值 Mean MNND randomization	MNND平均实际测量值 Mean MNND analysis	标准差 SD	最近邻体指数 NTI	零模型 Null model
10 m×10 m	172.63	238.72	55.80	-1.110	约束型 Constrained
10 m×10 m	231.88	238.72	89.65	0.007	非约束型 Unconstrained
20 m×20 m	141.09	199.81	49.40	-1.170	约束型 Constrained
20 m×20 m	188.50	199.81	74.82	-0.024	非约束型 Unconstrained
25 m×25 m	133.57	189.81	47.05	-1.180	约束型 Constrained
25 m×25 m	179.78	189.81	69.60	-0.034	非约束型 Unconstrained

尺度 Scale (m)	模型 Model	χ²	df	P
10 m×10 m	NRI vs. DBH	30.19	2	<0.0010
10 m×10 m	NTI vs. DBH	34.50	2	<0.0010
20 m×20 m	NRI vs. DBH	3.92	2	0.1409
20 m×20 m	NTI vs. DBH	14.78	2	0.0006
25 m×25 m	NRI vs. DBH	7.15	2	0.0280
25 m×25 m	NTI vs. DBH	12.64	2	0.0020
径级 DBH class
A (1 cm≤DBH< 5 cm)	NRI vs. scale	0.93	2	0.6384
A (1 cm≤DBH< 5 cm)	NTI vs. scale	6.51	2	0.0386
B (5 cm≤DBH<10 cm)	NRI vs. scale	3.69	2	0.1574
B (5 cm≤DBH<10 cm)	NTI vs. scale	0.81	2	0.6671
C (DBH≥10 cm)	NRI vs. scale	5.85	2	0.0538
C (DBH≥10 cm)	NTI vs. scale	2.61	2	0.2712

Functional characterization of the Arabidopsis SERRATE under salt stress

Functional characterization of the Arabidopsis SERRATE under salt stress

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