Diversity in seed oil content and fatty acid composition in Acer species with potential as sources of nervonic acid

doi:10.1016/j.pld.2020.10.003

Plant Diversity ›› 2021, Vol. 43 ›› Issue (01): 86-92.DOI: 10.1016/j.pld.2020.10.003

• Articles • 上一篇

Diversity in seed oil content and fatty acid composition in Acer species with potential as sources of nervonic acid

Xing He^a,d, De-Zhu Li^b, Bo Tian^a,c

a Key Laboratory of Sustainable Utilization of Tropical Plant Resources, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 88 Xuefu Road, Kunming, Yunnan, 650223, China;
b Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming, Yunnan, 650201, China;
c Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, 666303, China;
d University of Chinese Academy of Sciences, Beijing, 100049, China

收稿日期:2020-07-27 修回日期:2020-10-21 出版日期:2021-02-25 发布日期:2021-03-25
通讯作者: Xing He, De-Zhu Li, Bo Tian
基金资助:
We thank the Germplasm Bank of Wild Species in Southwest China and Kunming Botanical Garden, Chinese Academy of Sciences for their help in seed material collection. We thank Gong Xun, Shen Zongfang and Li Jiangying for their assistance in seed collection and the Tang Mingyong for his assistance in oil content detection. We also would like to thank the reviewers and editor for their comments and suggestions, and Editage (www.editage.com) for English language editing. This work was supported by the National Natural Science Foundation of China (grant number 31671732).

Diversity in seed oil content and fatty acid composition in Acer species with potential as sources of nervonic acid

Xing He^a,d, De-Zhu Li^b, Bo Tian^a,c

a Key Laboratory of Sustainable Utilization of Tropical Plant Resources, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 88 Xuefu Road, Kunming, Yunnan, 650223, China;
b Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming, Yunnan, 650201, China;
c Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, 666303, China;
d University of Chinese Academy of Sciences, Beijing, 100049, China

Received:2020-07-27 Revised:2020-10-21 Online:2021-02-25 Published:2021-03-25
Contact: Xing He, De-Zhu Li, Bo Tian
Supported by:
We thank the Germplasm Bank of Wild Species in Southwest China and Kunming Botanical Garden, Chinese Academy of Sciences for their help in seed material collection. We thank Gong Xun, Shen Zongfang and Li Jiangying for their assistance in seed collection and the Tang Mingyong for his assistance in oil content detection. We also would like to thank the reviewers and editor for their comments and suggestions, and Editage (www.editage.com) for English language editing. This work was supported by the National Natural Science Foundation of China (grant number 31671732).

摘要/Abstract

摘要： Nervonic acid (NA, cis-15-tetracosenoic acid) is a very long-chain monounsaturated fatty acid that has been shown to be a core component of nerve fibers and nerve cells. It can be used to treat and prevent many neurological diseases. At present, commercially available NA is mainly derived from Acer truncatum seeds, which contain about 5%-6% NA in their seed oil. The aim of this study were to identify and analyze NA-containing Acer species that could be used as NA resource plants. For this purpose, 46 Acer species seeds were collected in China and in some or all of the seed oils from these species 15 fatty acids were detected, including linoleic acid, oleic acid (C18:1^△9, C18:1^△11), erucic acid, palmitic acid, NA, linolenic acid (C18:3^△6,9,12, C18:3^△9,12,15), eicosenoic acid (C20:1^△11, C20:1^△13), stearic acid, behenic acid, tetracosanoic acid, arachidic acid, and docosadienoic acid. Nervonic acid was detected in all samples, but the content was highly variable among species. NA content over 9% was detected in eleven species, of which Acer elegantulum had the highest levels (13.90%). The seed oil content, seed weight, and fatty acid profiles varied among species, but the comprehensive evaluation value (W) showed that A. coriaceifolium could be a new potential NA resources plant. The results also showed that NA was significantly negatively correlated with palmitic acid, oleic acid, and eicosenoic acid, but positively correlated with eicosadienoic acid, behenic acid, erucic acid, and tetracosanoic acid, which indicate the probable pathway for NA biosynthesis in Acer plants. This study has identified Acer species that may serve as NA resources and will help guide subsequent species breeding programs.

关键词: Acer, Nervonic acid, Fatty acid, Seed oil, Seed weight

Abstract: Nervonic acid (NA, cis-15-tetracosenoic acid) is a very long-chain monounsaturated fatty acid that has been shown to be a core component of nerve fibers and nerve cells. It can be used to treat and prevent many neurological diseases. At present, commercially available NA is mainly derived from Acer truncatum seeds, which contain about 5%-6% NA in their seed oil. The aim of this study were to identify and analyze NA-containing Acer species that could be used as NA resource plants. For this purpose, 46 Acer species seeds were collected in China and in some or all of the seed oils from these species 15 fatty acids were detected, including linoleic acid, oleic acid (C18:1^△9, C18:1^△11), erucic acid, palmitic acid, NA, linolenic acid (C18:3^△6,9,12, C18:3^△9,12,15), eicosenoic acid (C20:1^△11, C20:1^△13), stearic acid, behenic acid, tetracosanoic acid, arachidic acid, and docosadienoic acid. Nervonic acid was detected in all samples, but the content was highly variable among species. NA content over 9% was detected in eleven species, of which Acer elegantulum had the highest levels (13.90%). The seed oil content, seed weight, and fatty acid profiles varied among species, but the comprehensive evaluation value (W) showed that A. coriaceifolium could be a new potential NA resources plant. The results also showed that NA was significantly negatively correlated with palmitic acid, oleic acid, and eicosenoic acid, but positively correlated with eicosadienoic acid, behenic acid, erucic acid, and tetracosanoic acid, which indicate the probable pathway for NA biosynthesis in Acer plants. This study has identified Acer species that may serve as NA resources and will help guide subsequent species breeding programs.

Key words: Acer, Nervonic acid, Fatty acid, Seed oil, Seed weight

Xing He, De-Zhu Li, Bo Tian. Diversity in seed oil content and fatty acid composition in Acer species with potential as sources of nervonic acid[J]. Plant Diversity, 2021, 43(01): 86-92.

图/表 4

参考文献 29

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中国濒危动物红皮书(1998) China Red Data Book (1998)	中国物种红色名录(2004) China Species Red List (2004)
	绝灭 Extinct (EX)
野生绝迹 Extinct in wild (EX)	野外绝灭 Extinct in Wild (EW)
国内绝迹 Extirpated (ET)	地区绝灭 Regionally Extinct (RE)
	极危 Critically Endangered (CR)
濒危 Endangered (E)	濒危 Endangered (EN)
易危 Vulnerable (V)	易危 Vulnerable (VU)
稀有 Rare (R)	近危 Near Threatened (NT)
	无危 Least Concern (LC)
未定 Indeterminate (I)	数据缺乏 Data Deficient (DD)
	不宜评估 Not Applicable (NA) 未予评估 Not Evaluated (NE)

中国濒危动物红皮书(1998) China Red Data Book (1998)	中国物种红色名录(2004) China Species Red List (2004)
	绝灭 Extinct (EX)
野生绝迹 Extinct in wild (EX)	野外绝灭 Extinct in Wild (EW)
国内绝迹 Extirpated (ET)	地区绝灭 Regionally Extinct (RE)
	极危 Critically Endangered (CR)
濒危 Endangered (E)	濒危 Endangered (EN)
易危 Vulnerable (V)	易危 Vulnerable (VU)
稀有 Rare (R)	近危 Near Threatened (NT)
	无危 Least Concern (LC)
未定 Indeterminate (I)	数据缺乏 Data Deficient (DD)
	不宜评估 Not Applicable (NA) 未予评估 Not Evaluated (NE)

中文名 Chinese name	拉丁名 Latin name	评估起始年份 Start year of period	评估结束年份 End year of period	评估起始年份濒危等级 Category at start of period	评估结束年份濒危等级 Category at end of period
兽类
白头叶猴	Trachypithecus poliocephalus	1996	2008	EN	CR
黑长臂猿	Nomascus concolor	1996	2008	EN	CR
双峰驼	Camelus bactrianus	1996	2008	EN	CR
林麝	Moschus berezovskii	1996	2008	NT	EN
黑麝	Moschus fuscus	1996	2008	NT	EN
豺	Cuon alpinus	1996	2008	VU	EN
蒙古野驴	Equus hemionus	1996	2008	VU	EN
伊犁鼠兔	Ochotona iliensis	1996	2008	VU	EN
藏羚羊	Pantholops hodgsonii	1996	2008	VU	EN
川金丝猴	Rhinopithecus roxellana	1996	2008	VU	EN
巨松鼠	Ratufa bicolor	1996	2008	LC	NT
鸟类
青头潜鸭	Aythya baeri	1994	2000	VU	EN
青头潜鸭	Aythya baeri	2004	2008	EN	CR
朱鹮	Nipponia nippon	1994	2000	CR	EN
黑脸琵鹭	Platalea minor	1994	2000	CR	EN
栗头鳽	Gorsachius goisagi	1988	1994	VU	EN
卷羽鹈鹕	Pelecanus crispus	2000	2004	NT	VU
黄爪隼	Falco naumanni	1994	2000	VU	NT
黄爪隼	Falco naumanni	2000	2004	NT	LC
猎隼	Falco cherrug	2000	2004	LC	VU
猎隼	Falco cherrug	2008	2012	VU	EN
黑兀鹫	Sarcogyps calvus	2000	2004	NT	CR
波斑鸨	Chlamydotis macqueenii	1994	2000	LC	NT
波斑鸨	Chlamydotis macqueenii	2000	2004	NT	VU
白头鹤	Grus monacha	1994	2000	NT	VU
白腰杓鹬	Numenius arquata	1994	2000	LC	NT
勺嘴鹬	Eurynorhynchus pygmeus	1994	2000	VU	EN
勺嘴鹬	Eurynorhynchus pygmeus	2004	2008	EN	CR
遗鸥	Larus relictus	1994	2000	NT	VU
中亚鸽	Columba eversmanni	1988	1994	NT	VU
栗斑腹鹀	Emberiza jankowskii	2000	2004	VU	EN
黄胸鹀	Emberiza aureola	1994	2000	NT	VU
淡水鱼类
施氏鲟	Acipenser schrencki	1998	2004	VU	EN
达氏鲟	Acipenser dabryanus	1998	2004	VU	EN
中华鲟	Acipenser sinensis	1998	2004	VU	EN
鳇	Huso dauricus	1998	2004	VU	EN
白鲟	Psephurus gladius	1998	2004	EN	CR
黑线	Atrilinea roulei roulei	1998	2004	NT	VU
须鳊	Pogobrama barbatula	1998	2004	NT	VU
海南	Hainania serrata	1998	2004	NT	VU
小似鲴	Xenocyprinoides parvulus	1998	2004	NT	VU
裸腹盲鲃	Typhlobarbus nudiventris	1998	2004	NT	VU
表2 (续) Table 2 (continued)
中文名 Chinese name	拉丁名 Latin name	评估起始年份 Start year of period	评估结束年份 End year of period	评估起始年份濒危等级 Category at start of period	评估结束年份濒危等级 Category at end of period
红鳍方口鲃	Cosmochilus cardinalis	1998	2004	NT	VU
袋唇鱼	Balantiocheilus hekouensis	1998	2004	NT	VU
暗色唇鲮	Semilabeo obscurus	1998	2004	NT	VU
华缨鱼	Sinocrossocheilus guizhouensis	1998	2004	NT	VU
缺须盆唇鱼	Placocheilus cryptonemus	1998	2004	NT	VU
长须片唇鮈	Platysmacheilus longibarbatus	1998	2004	NT	VU
塔里木裂腹鱼	Schizothorax biddulphi	1998	2004	EN	CR
鲃鲤	Puntioplites proctozysron	1998	2004	NT	VU
无眼岭鳅	Oreonectes anophthalmus	1998	2004	NT	VU
个旧盲高原鳅	Triplophysa gejiuensis	1998	2004	NT	VU
保亭近腹吸鳅	Plesiomyzon baotingensis	1998	2004	NT	VU
厚唇原吸鳅	Protomyzon pachycheilus	1998	2004	NT	VU
湄南缺鳍鲶	Kryptopterus moorei	1998	2004	NT	VU
长丝鱼芒	Pangasius sanitwangsei	1998	2004	NT	VU
长丝黑鮡	Gagata dolichonema	1998	2004	NT	VU
短须粒鲶	Akysis brachybarbatus	1998	2004	NT	VU
高体白甲鱼	Onychostoma alticorpus	1998	2004	NT	VU

中文名 Chinese name	拉丁名 Latin name	评估起始年份 Start year of period	评估结束年份 End year of period	评估起始年份濒危等级 Category at start of period	评估结束年份濒危等级 Category at end of period
兽类
白头叶猴	Trachypithecus poliocephalus	1996	2008	EN	CR
黑长臂猿	Nomascus concolor	1996	2008	EN	CR
双峰驼	Camelus bactrianus	1996	2008	EN	CR
林麝	Moschus berezovskii	1996	2008	NT	EN
黑麝	Moschus fuscus	1996	2008	NT	EN
豺	Cuon alpinus	1996	2008	VU	EN
蒙古野驴	Equus hemionus	1996	2008	VU	EN
伊犁鼠兔	Ochotona iliensis	1996	2008	VU	EN
藏羚羊	Pantholops hodgsonii	1996	2008	VU	EN
川金丝猴	Rhinopithecus roxellana	1996	2008	VU	EN
巨松鼠	Ratufa bicolor	1996	2008	LC	NT
鸟类
青头潜鸭	Aythya baeri	1994	2000	VU	EN
青头潜鸭	Aythya baeri	2004	2008	EN	CR
朱鹮	Nipponia nippon	1994	2000	CR	EN
黑脸琵鹭	Platalea minor	1994	2000	CR	EN
栗头鳽	Gorsachius goisagi	1988	1994	VU	EN
卷羽鹈鹕	Pelecanus crispus	2000	2004	NT	VU
黄爪隼	Falco naumanni	1994	2000	VU	NT
黄爪隼	Falco naumanni	2000	2004	NT	LC
猎隼	Falco cherrug	2000	2004	LC	VU
猎隼	Falco cherrug	2008	2012	VU	EN
黑兀鹫	Sarcogyps calvus	2000	2004	NT	CR
波斑鸨	Chlamydotis macqueenii	1994	2000	LC	NT
波斑鸨	Chlamydotis macqueenii	2000	2004	NT	VU
白头鹤	Grus monacha	1994	2000	NT	VU
白腰杓鹬	Numenius arquata	1994	2000	LC	NT
勺嘴鹬	Eurynorhynchus pygmeus	1994	2000	VU	EN
勺嘴鹬	Eurynorhynchus pygmeus	2004	2008	EN	CR
遗鸥	Larus relictus	1994	2000	NT	VU
中亚鸽	Columba eversmanni	1988	1994	NT	VU
栗斑腹鹀	Emberiza jankowskii	2000	2004	VU	EN
黄胸鹀	Emberiza aureola	1994	2000	NT	VU
淡水鱼类
施氏鲟	Acipenser schrencki	1998	2004	VU	EN
达氏鲟	Acipenser dabryanus	1998	2004	VU	EN
中华鲟	Acipenser sinensis	1998	2004	VU	EN
鳇	Huso dauricus	1998	2004	VU	EN
白鲟	Psephurus gladius	1998	2004	EN	CR
黑线	Atrilinea roulei roulei	1998	2004	NT	VU
须鳊	Pogobrama barbatula	1998	2004	NT	VU
海南	Hainania serrata	1998	2004	NT	VU
小似鲴	Xenocyprinoides parvulus	1998	2004	NT	VU
裸腹盲鲃	Typhlobarbus nudiventris	1998	2004	NT	VU
表2 (续) Table 2 (continued)
中文名 Chinese name	拉丁名 Latin name	评估起始年份 Start year of period	评估结束年份 End year of period	评估起始年份濒危等级 Category at start of period	评估结束年份濒危等级 Category at end of period
红鳍方口鲃	Cosmochilus cardinalis	1998	2004	NT	VU
袋唇鱼	Balantiocheilus hekouensis	1998	2004	NT	VU
暗色唇鲮	Semilabeo obscurus	1998	2004	NT	VU
华缨鱼	Sinocrossocheilus guizhouensis	1998	2004	NT	VU
缺须盆唇鱼	Placocheilus cryptonemus	1998	2004	NT	VU
长须片唇鮈	Platysmacheilus longibarbatus	1998	2004	NT	VU
塔里木裂腹鱼	Schizothorax biddulphi	1998	2004	EN	CR
鲃鲤	Puntioplites proctozysron	1998	2004	NT	VU
无眼岭鳅	Oreonectes anophthalmus	1998	2004	NT	VU
个旧盲高原鳅	Triplophysa gejiuensis	1998	2004	NT	VU
保亭近腹吸鳅	Plesiomyzon baotingensis	1998	2004	NT	VU
厚唇原吸鳅	Protomyzon pachycheilus	1998	2004	NT	VU
湄南缺鳍鲶	Kryptopterus moorei	1998	2004	NT	VU
长丝鱼芒	Pangasius sanitwangsei	1998	2004	NT	VU
长丝黑鮡	Gagata dolichonema	1998	2004	NT	VU
短须粒鲶	Akysis brachybarbatus	1998	2004	NT	VU
高体白甲鱼	Onychostoma alticorpus	1998	2004	NT	VU

Diversity in seed oil content and fatty acid composition in Acer species with potential as sources of nervonic acid

Diversity in seed oil content and fatty acid composition in Acer species with potential as sources of nervonic acid

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